DNA sequencing, nested polymerase chain reaction (PCR), and PCR-restriction fragment length polymorphism were used to investigate mutations in the coding regions of the bovine myeloid antimicrobial peptide-28 (BMAP-28) and mannan-binding lectin-associated serine protease-2 (MASP-2) genes in 249 Chinese Holstein dairy cows. The associations between the polymorphisms and somatic cell score (SCS) ...

Data from six experiments (two with dry cows) were used to predict partitioning of gross energy to CH4 in Holstein cows using selected independent variables, some of which were intercorrelated, and a stepwise backward elimination regression procedure. Methane outputs ranged from 3.1 to 8.3% (mean 5.5) of gross energy intake for 134 dry cow balance trials and from 1.7 to 14.9% (mean 5.2) of gros...

The aim of the present study was to investigate the changing profiles of plasma metabolic hormones during the ovarian cycles of beef and dairy cattle. We used 16 non-pregnant, non-lactating Japanese Black beef cattle (6 heifers and 10 cows; parity=2.3 +/- 0.8) and 12 multiparous Holstein dairy cows (parity=3.0 +/- 0.3). Blood samples for hormonal analysis (growth hormone, GH; insulin-like growt...

 In US dairy herds, the current national average age at first calving is 25.2 months. Increasing first-lactation milk yield could improve milk’s life-cycle production efficiency and decrease emissions.  The productive life of dairy Holsteins in the United States born in 2000 decreased by 3.95 months compared with Holstein cows born in 1980. This negative trend needs to be halted to achieve en...

The objective of this study was to investigate the effect of a partial substitution of soybean meal with field pea (Pisum sativum) in Holstein lactating dairy cow diets on dry matter intake, milk yield and composition, blood metabolites, rumen ammonia-N concentration and pH. Eighteen lactating Holstein cows were randomly assigned into three groups based on parity, days in milk, and mil...

The objective of this study was to determine how the concentration of milk urea (MU) and FPCM production affect reproduction in selected Holstein dairy herds in the Czech Republic. A retrospective, observational study comprising analyses of individual cow records from monthly dairy herd improvement (DHI) milk tests including milk urea (MU) concentration was conducted in six commercial Holstein ...

The aim of this study was to determine if, and under what conditions, indoor-housed dairy cows would seek a shelter to calve. Seventy-two Holstein dairy cows were paired by expected calving date and moved into a maternity pen that contained an open area with no cover and a sheltered area that was covered on all sides except for the ceiling and an entrance where cows could freely enter or exit. ...

The association between blood calcium levels and electrocardiographic variables was compared in 137 normal parturient and 36 peripartum recumbent Holstein cows to determine whether hypocalcemia in peripartum dairy cows can be rapidly diagnosed using electrocardiograph. Inverse of STc (ST peak interval/SS interval(0.5)) and blood ionized calcium or serum calcium concentrations were strongly corr...

the aim of this study was to consider reproductive performance parameters and blood progesterone concentration changes in high-producing dairy cows (39±2 kg per day) after artificial insemination, in summer and winter. 270 heads of holstein dairy cattle were choose in three big dairy farms, and blood samples were collected at days seven, ten and fourteen after artificial insemination. temperatu...

OBJECTIVE The aim of the current study was to describe the relationship between milk yield and lactation number, stage, length and milking frequency in Korean Holstein dairy cows using an automatic milking system (AMS). METHODS The original data set consisted of observations from April to October 2016 of 780 Holstein cows, with a total of 10,751 milkings. Each time a cow was milked by an AMS ...