Enzyme histochemistry serves as a link between biochemistry and morphology. It is based on metabolization of a substrate provided to a tissue enzyme in its orthotopic localization. Visualization is accomplished with an insoluble dye product. It is a sensitive dynamic technique that mirrors even early metabolic imbalance of a pathological tissue lesion, combined with the advantage of histotopogr...

Nitric oxide (NO) has been implicated in neurotoxicity and cerebral blood flow changes in chronic neurodegeneration, but its activity in the mammalian prion diseases has not been studied in detail. Nicotine adenine dinucleotide phosphate (NADPH)-diaphorase (NADPH-d) histochemistry is a simple and robust histochemical procedure that allows localization of the tissue distribution of NO synthases....

Recently orotic acid has been noticed as a growth factor and regarded as a pre cursor of nucleic acid (1, 2). But its distribution in organisms has scarcely been studied except for tracing the acid using C14-labeled compound (3). The authors have found that orotic acid shows a whitish blue primary fluorescence, which is re markably intensified by adding H2O2. Applying this property to histochem...

Background: Infertility problem affects young couples. One of the known causes of spermatogenic disorder is chemotherapy in patients with cancer. Since dividing cells are mainly affected by anticancer drugs, the aim of the present study is to investigate the preventive effect of GnRH antagonist on spermatogenic cell apoptosis produced by anticancer drug. Materials and Methods: In the present st...

The soils developed under High Altitude Rocky Complexes in Brazil are generally of very low chemical fertility, with low base saturation and high exchangeable aluminium concentration. This stressful condition imposes evolutionary pressures that lead to ecological success of plant species that are able to tolerate or accumulate high amounts of aluminium. Several analytical methods are currently ...

I . A histochemical study has been made of the cytoplasmic inclusions of the epithelial cells lining the storage section of the canal in the head of the epididymis of the mouse. 2. Spherical bodies, usually situated in the vicinity of the nucleus and here referred to as juxta-nuclear bodies, are shown to consist of cerebroside with some phospholipid. The juxta-nuclear bodies have been regarded ...

Integument from blue and green areas of the variant blue frog were analyzed biochemically for pteridines and carotenoids. Solvent extraction and absorption spectrophotometry indicated that p carotene was greatly reduced in the blue skin, and present in high quantities in the green skin of the blue frog. Thin layer and paper chromatography indicated that the pteridines were almost totally lackin...