The physical and chemical properties of six crude phytase preparations were compared. Four of these enzymes (Aspergillus A, Aspergillus R, Peniophora and Aspergillus T) were produced at commercial scale for the use as feed additives while the other two (E. coli and Bacillus) were produced at laboratory scale. The encoding genes of the enzymes were from different microbial origins (4 of fungal o...

This study examined the feasibility of using the promoter of the pig parotid secretory protein (PSP) gene for expression of the phytase transgene in mouse models. The pig parotid secretory protein gene is specifically expressed at high levels in the salivary glands. The 10-kb upstream promoter region of the gene necessary for tissue-specific expression has been identified. We have constructed p...

Two studies were conducted to determine the true P digestibility (TPD) of black-eyed pea (BEP) and peanut flour (PNF) and the TPD response to phytase supplementation using the regression method. Sequential diets containing 115, 230, or 345 g of BEP/kg (experiment 1) and 115, 230, or 345 g of PNF/kg (experiment 2) without or with 1,000 units of phytase/kg were formulated. Chromic oxide was added...

Supplementation with phytase is an effective way to increase the availability of phosphorus in seed-based animal feed. The biochemical characteristics of an ideal phytase for this application are still largely unknown. To extend the biochemical characterization of wild-type phytases, the catalytic properties of a series of fungal phytases, as well as Escherichia coli phytase, were determined. T...

introduction : methotrexate (mtx) is an anti folate drug that used in malignant and non malignant patients. the usage of high dose methotrexate was limited to patients with: osteogenic sarcoma, ewing sarcoma and lymphoma. the aim this study was to determine the toxicity or side effects of very high dose methotrexate (8-10 gr/m 2 /cycle). this study is the first study in iranian patients and one...

Traditional supplemental dietary phytase loses activity during steam pelleting. The thermal tolerance and bioefficacy of a phytase product with a thermoprotective coating [coated phytase (C-phytase)] was compared in mash and pelleted diets to a traditional, uncoated phytase (U-phytase) added to a negative control (NC) diet, formulated with reduced dietary Ca and P, and compared with a corn-soyb...

Three experiments were conducted to test the hypotheses that pigs fed diets that are equal in digestible P will perform equally regardless of the concentration of total P in the diets, and that the addition of microbial phytase, distillers dried grains with solubles (DDGS), or a combination of phytase and DDGS will result in a reduction in P excretion. In Exp. 1, a P-free diet and 6 diets conta...

The use of a suitable methodology to quantify the phytate phosphorus (Pphy) content in both the feed and the excreta from broilers is required to enable accurate calculation of the catalytic efficiency of the phytase supplemented in the feed. This study was conducted to compare 2 analytical methodologies (colorimetry and also high-performance liquid chromatography with a refractive index detect...

Two studies with growing pigs were conducted using the regression analysis technique to estimate the true digestibility of P (TPD) in canola (CM) and soybean (SBM) meals, and quantify the effect of microbial phytase on TPD of CM and SBM. In each study, 48 (Exp. 1) or 36 (Exp. 2) 17-kg barrows were assigned to 6 dietary treatments arranged in a 3 x 2 factorial of 3 graded levels of CM (Exp. 1) o...

The inclusion of phytase in monogastric animal feed has the benefit of hydrolyzing indigestible plant phytate (myo-inositol 1,2,3,4,5,6-hexakis dihydrogen phosphate) to provide poultry and swine with dietary phosphorus. An ideal phytase supplement should have a high temperature tolerance, allowing it to survive the feed pelleting process, a high specific activity at low pHs, and adequate gastri...