High density lipoprotein has anti-inflammatory effects in addition to mediating reverse cholesterol transport. While many of the chronic anti-inflammatory effects of high density lipoprotein (HDL) are attributed to changes in cell adhesion molecules, little is known about acute signal transduction events elicited by HDL in endothelial cells. We now show that high density lipoprotein decreases e...

in order to increase the productivity of human interleukin-2 (il-2) as a model protein in the recombinant e.colibl21 (de3), the effect of magnesium acetate and manganese acetate was studied based on full factorial design experiments magnesium acetate and manganese acetate and their interaction exhibited an increasing effect on il-2 expression level upto 25.24%, 16.80% and 23.30%, respectively, ...

Pure culture of magnetotactic bacteria with high magnetosome yield has been achieved for only a few strains. The major obstacles involve the nutritional requirements and culture conditions of the cells. To increase cell density and magnetosome production, it is necessary to elucidate the physiological characteristics of a particular strain during cell growth and develop an appropriate artificia...

the present study investigates the effect of different exposure densities on third grade iranian junior high school (ijhs) students’ efl development and retention. it also attempts to explore whether there is any differential performance due to type of post-exposure assessment tasks (recognition vs. written production). performances of five groups of learners receiving equal amount of exposure ...

hippocampal neural stem/progenitor cells (hipp-ns/pcs) of the adult mammalian brain are important sources of neuronal and gial cell production. in this study, the main goal is to investigate the plasticity of these cells in neuronal/astroglial differentiations. to this end, the differentiation of the hipp-ns/pcs isolated from 3-month-old wistar rats was investigated in response to the embryonic...

Regulation of energy balance in photosystems in response to extremely-high-CO2 (40%) and low-CO2 (0.04%) stress was studied in extremely-high-CO2-tolerant green microalgae, Chlorococcum littorale and Chlorella sp. UK001. To investigate the energy input process, we assessed an F714/F685-ratio in a 77K fluorescence emission spectrum induced by 440-nm excitation in intact cells, which represents a...

Cell retention devices are used to retain cells in containers during perfusion culture. However, the conventional membrane-based filtration devices have challenges such as membrane fouling/clogging and increased contamination risk due to frequent filter replacements. To solve these challenges, we introduce a new microfluidic cell retention device based on inertial cell focusing. We demonstrated...

lipofectamine ltx is a cationic based transfection reagent that offers high levels of different transgenes expression in a huge number of cell types in vitro utilizing an easy and rapid protocol. the level of transgenes expression depend on some experimental variables containing cell density, lipofectamine and dna concentrations, lipofectamine -dna complexing time, and the presence or absence o...

Expression of foreign proteins in E. coli is normally inhibited by exogenous production of acetate. To overcomethis problem, various strategies have been proposed and tested to reduce the extent of acetate accumulation.Although these strategies can improve the outcome, the implementation of their proposed techniquesis not practical. Because to achieve optimal results, it requi...