Incidence of the loss of heterozygosity on chromosome 3p was evaluated using 7 polymorphic probes in 35 Japanese patients with sporadic renal cell carcinoma (RCC). Overall frequency of the loss of heterozygosity on 3p was 53%, representing 16 of 30 informative cases. Examination of the relationship between histopathological phenotypes of RCC and incidence of the 3p loss revealed that the loss o...

The main objective of this study was to assess genetic comparison of two migratory forms of Caspian trout Salmo trutta caspius namely fall-run and spring-run. Owing to the lack of information on its genetic differences, 5 microsatellite loci were used for 58 sample analyses. Genomic DNA was extracted from caudal fin using Roche DNA extraction kit and each PCR reaction was performed in a 25 ?l ...

Parentage testing is an important tool in farm animals for genetically determining the accuracy of pedigree information. The objective of the current study was to implication of multiplexing 14 microsatellite markers for routine parentage testings. The twenty-four lambs were crossbred of Ghazel × Baluchi, Ghazel × Baluchi × Merinos, and Baluchi × Moghani × Merinos breeds. The genomic DNA was ex...

Loss of heterozygosity on chromosomal arm 16q has been shown to be a frequent event in sporadic breast cancer and is suggested to be involved in cancer development through inactivation of a tumor-suppressor gene. To specify the commonly deleted region in which the unknown tumor-suppressor gene is located, a deletion map of chromosome 16 was constructed for 78 breast cancers, using 27 polymorphi...

Although heterozygosity-fitness correlations (HFCs) are widely reported in the literature, most studies use too few markers to allow the proximate mechanisms to be convincingly resolved. Two competing hypotheses have been proposed: the general effect hypothesis, in which marker heterozygosity correlates with genome-wide heterozygosity and hence the inbreeding coefficient f, and the local effect...

Two RAPD primers and 15 protein biochemical markers were examined to assess the zygosity (hetero/homozygocity) and per cent polymorphisms in monogamous (IM: IF) and triogamous (1M:2F) breeding groups of outbred Mongolian gerbils (Meriones unguiculatus). No polymorphism and heterozygosity were found in RAPD as well as biochemical markers in monogamous groups, whereas both primers in RAPD (%P=55%...

Daphnia magna belongs to the Cladocera and plays an important role in water ecosystem. With intensification of pollution, wild population D. has declined rapidly recent years, insufficient molecular markers have limited effective research conservation this species. In research, 30 novel single nucleotide polymorphism (SNP) were developed a cultivar 12 using restriction site-associated DNA seque...