Heteronuclear dual-site catalysts (HDACs), inspired from single-atom catalyst, have been proposed as advanced alternatives of noble metal especially toward nitrogen reduction reaction (NRR). However, the search for favorable candidates with both promising NH3 yield and Faradaic efficiency is still challenging, due to limited experimentally available bi-metal pairs lack thorough understanding de...

In this paper, we present a series of heteronuclear NMR experiments for the direct observation and characterization of lysine NH3 groups in proteins. In the context of the HoxD9 homeodomain bound specifically to DNA we were able to directly observe three cross-peaks, arising from lysine NH3 groups, with 15N chemical shifts around approximately 33 ppm at pH 5.8 and 35 degrees C. Measurement of w...