The recombinant ovalbumin (OVA) produced in yeast Pichia pastoris was purified from the culture medium by anion exchange chromatography, and its structural characteristics were compared with those of hen egg OVA, mainly from the point of view of posttranslational modification. The expressed OVA consisted of two molecular species immmunoreactive with antibody for hen egg OVA. The two molecular s...

The target size of neurotoxic esterase (NTE), the putative target site for the initiation of organophosphorus-compound-induced delayed neurotoxicity, and acetylcholinesterase (AChE) from hen brain were examined by determining the rate at which the activities of the esterases were destroyed by ionizing irradiation. Samples of hen brain were prepared by slowly drying a microsomal preparation unde...

This is a Health Evidence Network (HEN) synthesis report on injuries in children and older people. The evidence of proven and promising strategies for the prevention of unintentional injuries in children and the older people is considerable. Legislative, environmental modification and educational approaches all have a part to play in preventing or reducing childhood injuries, and their interact...

Heat inactivation was studied at 45, 50, 55, and 60 degrees for all of the phenyl valerate hydrolases (PVase), including neurotoxic esterase (NTE) and inhibitor-resistant esterase (IRE), in homogenates of hen or rat brain or in preparations of hen brain microsomal membranes. Hen and rat brain homogenates were prepared in buffer (50 mM Tris/0.20 mM EDTA, pH 8.00, at 25 degrees). Hen brain micros...

Optimization of carnosine and anserine extraction from chicken breast was performed using response surface methodology (RSM) to obtain the maximized physiological activities for anti-glycation and anti-oxidation. The optimum extraction conditions were water extraction for 1.6 h in the case of the 20-wk laying hen muscle and water extraction for 2.12 h in the case of 90-wk laying hen muscle. Hig...

Samenvatting Economisch daklozen zijn mensen die vaak wel een baan maar geen huis hebben. Ze zoeken met spoed woonruimte. Op de huidige krappe woningmarkt er voor hen mogelijkheden en in opvang is plaats. In vier praktijkgevallen – drie steden regio wordt gezocht naar oplossingen deze groep mensen. Dat lukt, op kleine schaal. Voor hen, ook andere spoedzoekers, biedt ander, aanvullend type wonin...

Toll-like receptors (TLR) activate multiple steps in inflammatory reactions in innate immune responses. They also activate signals that are critically involved in the initiation of adaptive immune responses. Many tumorigenic chemicals have been associated with endotoxin hypersensitivity mediated through TLR4. To determine the role of TLR4 in cutaneous skin carcinogenesis, we treated TLR4-defici...

The organisation of services to support the increasing number of people receiving enteral tube feeding (ETF) at home varies across regions. There is evidence that multi-disciplinary primary care teams focussed on home enteral nutrition (HEN) can provide cost-effective care. This paper describes the development and evaluation of a HEN Team in one UK city. A HEN Team comprising dietetians, nurses...

In the domestic hen phenylacetic acid is excreted conjugated mainly with ornithine (Totani, 1910), but in the pigeon it is excreted conjugated with taurine and glycine (James et al., 1972). This difference between the hen and pigeon has now been further investigated with several arylacetic acids. The compounds used were 14C-carboxy-labelled phenylacetic acid, 4-chloroand 4-nitrophenylacetic aci...

It is well known that in the hen the egg-white protein ovotransferrin (conalbumin) and the serum protein transferrin are very similar in structure and iron-binding properties (for review see Beam & Parker, 1966). Since it is evident that for the eggwhite protein the name 'ovotransferrin' (Komatsu & Feeney, 1967; Azari & Baugh, 1967) is more informative than the classical name 'conalbumin', it i...