نتایج جستجو برای: hemocyanins
تعداد نتایج: 116 فیلتر نتایج به سال:
cDNA clones comprising the entire coding region of two out of the seven heterogeneous subunits of hemocyanin from the tarantula, Eurypelma californicum, were isolated from four cDNA libraries constructed from total RNA from the heart tissue of single spiders. Hybridization was first carried out using a tarantula hemocyanin subunit e partial cDNA, and several positive clones were isolated, inclu...
Cryptocyanin, a copper-free hexameric protein in crab (Cancer magister) hemolymph, has been characterized and the amino acid sequence has been deduced from its cDNA. It is markedly similar in sequence, size, and structure to hemocyanin, the copper-containing oxygen-transport protein found in many arthropods. Cryptocyanin does not bind oxygen, however, and lacks three of the six highly conserved...
In 1937 a protein material was isolated (1) by ultracentrifugation from extracts of warts (2) occurring naturally in Western cottontail rabbits. Readily purified by alternate low and high speed centrifugation the material sediments in the analytical ultracentrifuge with the sharp boundary indicative of high homogeneity and with a sedimentation constant determined only approximately in previous ...
Background: Genome walking is a DNA-cloning methodology that is used to isolate unknown genomic regions adjacent to known sequences. However, the existing genome-walking methods have their own limitations. Objectives: Our aim was to provide a simple and efficient genome-walking technology. Material and Methods: In this paper, we dev...
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