background: ferroportin (fpn), a regulator of iron homeostasis is a conserved membrane protein that exports iron across the enterocytes, macrophages and hepatocytes into the blood circulation. fpn has also critical influence on survival of microorganisms whose growth is dependent upon iron, thus preparation of fpn is needed to study the role of iron in immunity and pathogenesis of micoorganisms...

E46(+) virus purified by limiting dilution and plaque isolation in AGMK cells consists of a mixture of hybrid and non-hybrid virus particles. Of 102 plaque isolates obtained in HEK cells, 98 contained no detectable hybrid virus, while 4 contained a small proportion of hybrid virus. Plaque isolates from AGMK cells uniformly induced SV40 neoantigen, but when these plaque isolates were plaqued in ...

Glutathione-dependent formaldehyde dehydrogenase (GFD) from Taiwanofungus camphorata plays important roles in formaldehyde detoxification and antioxidation. The enzyme is bifunctional. In addition to the GFD activity, it also functions as an effective S-nitrosoglutathione reductase (GSNOR) against nitrosative stress. We investigated the modulation of HEK (human embryonic kidney) 293T cells unde...

47 48 We have characterized the native voltage-dependent K + (K v) current in rabbit urethral smooth 49 muscle cells (RUSMC) and compared its pharmacological and biophysical properties with 50 K v 2.1 and K v 2.2 channels cloned from the rabbit urethra and stably expressed in HEK 293 51 cells (HEK Kv2.1 and HEK Kv2.2). RUSMC were perfused with Hanks' solution at 37°C and 52 studied using the pa...