The storage of biological samples may affect detection of viral nucleic acid, yet the stability of viral nucleic acid at standard laboratory storage temperatures (-70°C and -20°C) has not been comprehensively assessed. Deterioration of viral RNA and DNA during storage may affect the detection of viruses, thus leading to an increased likelihood of false-negative results on diagnostic testing. Th...

BACKGROUND/AIMS Complex mutants may be selected under sequential anti-VHB pressures. We analyzed the genotypic and phenotypic evolution of the viral quasi-species of a patient who developed resistance to entecavir following lamivudine breakthrough. METHODS The polymerase gene was amplified, cloned and sequenced at different time points. Hepatoma cell lines were transfected to compare the repl...

BACKGROUND Complex mutants can be selected under sequential selective pressure by HBV therapy. To determine hepatitis B virus genomic evolution during antiviral therapy we characterized the HBV quasi-species in a patient who did no respond to therapy following lamivudine breakthrough for a period of 14 years. CASE PRESENTATION The polymerase and precore/core genes were amplified and sequenced...

BACKGROUND The chronicity of hepatitis B virus (HBV) infection is attributed to inappropriate functioning of cell-mediated immunity. Besides the importance of measuring serum HBV DNA and HBV surface antigen (HBsAg) as markers of viral replication and exposure, respectively, studies regarding their influence on immune cell status in chronic HBV infection are still scarce. Because such studies of...

BACKGROUND Selection of drug-resistant strains may lead to failure of HBV antiviral therapy. There is little information whether there is detection difference in drug resistant mutations between different viral load assays of HBV. OBJECTIVES This study is aimed to investigate whether there is drug-resistant strains related detection difference between Abbott RealTime HBV (RealTime) and CobasA...

This paper deals with a fractional optimal control problem model that describes the interactions between hepatitis B virus (HBV) HBV DNA-containing capsids, liver cells (hepatocytes), and cytotoxic T-cell immune response. Optimal controls represent effectiveness of drug therapy in inhibiting viral production preventing new infections. The optimality system is derived solved numerically. Our res...

The performance of the m1000 system (Abbott Laboratories, Illinois) as a front-end extraction system for high-throughput "in-house" quantitative real-time PCR assays was analyzed and compared to that of manual extraction of plasma and serum samples (hepatitis C virus [HCV] and hepatitis B virus [HBV]) and EDTA-blood samples (cytomegalovirus [CMV] and Epstein-Barr virus [EBV]). Linearity of extr...

Context: Characteristics and factors influencing viral decay under TDF and ADV need to be determined in HIV-HBV co-infected patients. Patients and methods: This non-randomized, open label study compared the HBV DNA decay in 85 HIV-HBV co-infected patients initiating a combined antiretroviral regimen either including TDF or associated with ADV. The first 6 monthchange in viral load was analyzed ...

BACKGROUND Although adherence is of major importance in long-term treatments, few studies have been published regarding the use of anti-HBV analogues in clinical practice. The aim of this study was to evaluate adherence to anti-HBV analogues and associated virological suppression. METHODS A cross-sectional study was performed between 1 January 2009 and 15 July 2009 in Cochin Hospital, Paris, ...

BACKGROUND Characteristics and factors influencing viral decay under tenofovir (TDF) and adefovir (ADV) need to be determined in HIV-HBV-coinfected patients. METHODS This open-label study compared the HBV dynamics in 85 HIV-HBV-coinfected patients initiating an antiretroviral regimen, either including TDF or associated with ADV. The first 6-month change in viral load was analysed using mixed ...