Fetal hemoglobin (Hb F) is characteristic of the fetal development period. However, in some genetic conditions, such as hereditary persistence of fetal hemoglobin (HPFH) and delta-beta thalassemia (δβ-thalassemia), Hb F continues to be produced in adulthood. We evaluated the frequency of two mutations of HPFH, HPFH-1 and HPFH-2 African, and two mutations in δβ-thalassemia, Sicilian and Spa...

We have measured the solubility of sickle hemoglobin by ultracentrifugation of concentrated solutions of deoxyhemoglobins S + A and S + F in 0.06 I phosphate, pH 7.0. Cyanmethemoglobin derivatives were deoxygenated, mixed, and reduced with dithionite in such a way as to produce gelled deoxyhemoglobin solutions containing 50% asymmetrical hybrid tetramers (a~3~/3* or CU#+~) and solutions contain...

Low-density lipoprotein (LDL) oxidation mediated by a variety of catalysts in atherosclerotic lesions plays a crucial role in the genesis and evolution of atherosclerotic plaques. In this study we focused on oxidative properties of hemoglobin (Hb)-modified LDL because Hb is present in atherosclerotic lesions. Under low oxygen tensions Hb was previously found to modify apolipoprotein B100 with c...

Abstract Background: Fecal immunochemical test (FIT) performance can be affected by post-collection variables. Collection technique might also affect fecal hemoglobin concentration (f-Hb). Variation in quantity of feces collected samples returned a colorectal cancer detection program, and the effects under-sampling, were assessed. Methods: devices obtained from patients undergoing FIT assessed ...

The role of retinoic acid receptors (RARs) in intercellular regulation of cell growth was assessed by targeting a dominant-negative RARalpha mutant (dnRARalpha) to differentiated suprabasal cells of mouse epidermis. dnRARalpha lacks transcriptional activation but not DNA-binding and receptor dimerization functions. Analysis of transgenic mice revealed that dnRARalpha dose-dependently impaired i...

hemoglobin f (hbf, α 2 γ 2 ) is a major contributor to the clinical heterogeneity and ameliorating agent observed in patients with the β-globin disorders including β-thalassemia and sickle cell disease (scd). during fetal life, hbf is the major hemoglobin but is largely substituted by adult hemoglobin (hba, α 2 β 2 ) following a globin expression switch after birth. increased γ-globin expressio...

H UMAN FETAL HEMOGLOBIN consists of two unlike pairs of polypeptide chains, i.e., a2y2. While the detection of an a-chain variant may not present serious difficulties, since the abnormal fetal hemoglobin will be replaced by the adult type, the y-chain variants are transitory and their study is limited by time, shortage of blood and their rarity. Identification of the y-chain anomaly has been po...

Aims. To compare HB&L and BACTEC systems for detecting the microorganisms contaminating the corneal storage liquid preserved at 31°C. Methods. Human donor corneas were stored at 4°C followed by preservation at 31°C. Samples of the storage medium were inoculated in BACTEC Peds Plus/F (aerobic microorganisms), BACTEC Plus Anaerobic/F (anaerobic microorganisms), and HB&L bottles. The tests were pe...

cell-mediated immune (cmi) s t a t us and sub- popul at i ons o f pe r ipheral b l ood lymphocytes were investigated in one hundre d volunt a ry blood donors who were car r ier s of ag • he s a signi f i c ant decr e ase of t otal t-cells observed in hb ag carri e rs as compared t o normal controls. the percens t age o f active t-cells a nd b-lymphocytes did not d i f f e r signi f icant ly bet...

The in uitm biosynthesis of acetylated human fetal hemoglobin (Hb FI) was investigated in umbilical cord blood from two normal newborns and in peripheral blood from five adults with different hematologic disorders accompanied by elevated levels of fetal hemoglobin. Hemoglobin biosynthesis was measured by [8HJleucine incorporation into hemoglobin components which were separated by chromatograph...