Doubled haploid technology has been used by various private companies. However, information regarding chromosome duplication methodologies, particularly those concerning techniques used to identify duplication in cells, is limited. Thus, we analyzed and characterized artificially doubled haploids using microsatellites molecular markers, pollen viability, and flow cytometry techniques. Evaluated...

The major component of complex genomes is repetitive elements, which remain recalcitrant to characterization. Using maize as a model system, we analyzed whole genome shotgun (WGS) sequences for the two maize inbred lines B73 and Mo17 using k-mer analysis to quantify the differences between the two genomes. Significant differences were identified in highly repetitive sequences, including centrom...

an efficient method for producing doubled haploid plants of date palm ( phoenix dactylifera l.) was established using in vitro colchicine treatment of anthers. to this study male inflorescences were harvested in the appropriate developmental stage. separated anthers after one day cold (4°c) pretreatment, in uninucleate stage of microspore were transferred to the modified y 3 medium containing d...

Haploids and doubled haploid (DH) inbred lines have become an invaluable tool for maize genetic research and hybrid breeding, but the genetic basis of in vivo induction of maternal haploids is still unknown. This is the first study reporting comparative quantitative trait locus (QTL) analyses of this trait in maize. We determined haploid induction rates (HIR) in testcrosses of a total of 1061 p...

Doubled haploids of carnation (Dianthus caryophyllus L.) were obtained by pseudofertilized ovule culture. Emasculated ̄ower buds of carnation were pollinated with pollen inactivated by Xray irradiation. After 2±3 weeks, the ovaries were explanted and were cultured on solid MS medium containing 2 mM NAA, 2 mM BAP and 6% sucrose. Regenerated plants(R0) were morphologically different from the moth...

Purpose. Investigation of cytogenetic aspects embryological processes in the culture immature apomictic embryos, breeding genotypes sugar beet with cytoplasmic sterility for differentiation and selection by gametophyte reduced parthenogenesis.
 Methods. Cytological, biotechnological, fluorescent cytophotometry, field, laboratory.
 Results. The features genesis embryos cells induced vi...

Wheat crop has a major role in current food system and global security. Beyond doubt, wheat production increased substantially, however, there been increase world’s population also, thereby, leading to supply demand. To fulfil this demand, science today needs more efficient new technologies for breeding with faster pace. Conventional is time consuming as it requires the selfing from F1 generati...