To separate the unknown Streptomyces strains isolated from soil samples, the interspacer regions of 16S-23S rDNA of 14 isolates were amplified with PCR (polymerase chain reaction) and digested with three restriction endonucleases, namely, Bsp143I, HaeIII and MnlI. The restriction patterns were used for RFLP (restriction fragment length polymorphism) analysis. A dendrogram were constructed using...

Fig. S1 CRISPR/Cas9-mediated genome editing in HepG2 Cells A) top, schematic depiction of CRISPR-targeted CpG island sequence. CRISPR guide sequences (gRNA-1 and gRNA-2) are highlighted in grey, and proto-spacer adjacent motif (PAM)s are underlined. A) bottom, the target region was eliminated in nonhomologous end joining DNA repair. Primer pairs used to determine the target deletion are indicat...

Restriction endonuclease analysis with HindIII, HaeIII, and BglII endonucleases of DNA extracted from each of eight colonies of Campylobacter pylori subcultured from a stomach biopsy and from each of eight colonies subcultured from dental plaque of the same patient indicated that at least three strains were present in the dental plaque but only one strain was present in the biopsy. One of the d...

The DNA fingerprinting of Helicobacter pylori strains in two cases of acute gastritis that occurred after endoscopy was examined. H. pylori was isolated from the stomachs of two patients with acute gastritis and from the stomachs of the patients in whom the same gastrofiberscope had previously been used. The genomic DNA digested with HaeIII was subjected to pulsed-field gel electrophoresis. The...

Mexico is considered endemic for Leishmania; recent reports indicate autochthonous human and canine leishmaniasis caused by Leishmania mexicana in Sinaloa state. Lutzomyia sand fly are the primary vector of parasite, although no records phlebotomine vectors exist from Sinaloa. Other hematophagous dipterans, like Culicoides, could represent possible absence phlebotomines. The known distribution ...

Genetic improvement of livestock productivity can be done through molecular selection on the genes controlling growth traits. polymorphism family (GH, GHRH, and PIT1) were studied in local swamp buffalo (106 hds.) from a government breeding station (46 heads) smallholders (60 North Tapanuli District, Sumatra Province. Genotype variants three identified by PCR-RFLP method using restriction enzym...

A total of 20 skin lesion samples collected from patients with cutaneous leishmaniasis (CL) attended AL-Karamah Teaching Hospital at Wasit province. Dermal scrapings were analysed by examination of Giemsa-stained smears, by Conventional PCR and RFLP-PCR methods. The incidence rate with CL was 80% by using Giemsa-stained and 100% for both PCR and RFLP-PCR. Twelve (60%) of isolates were typed as ...

Engineering the specificity of DNA-modifying enzymes has proven extremely challenging, as sequence recognition by these enzymes is poorly understood. Here we used directed evolution to generate a variant of HaeIII methyltransferase that efficiently methylates a novel target site. M.HaeIII methylates the internal cytosine of the canonical sequence GGCC, but there is promiscuous methylation of a ...

Genetic improvement programs may improve disease resistance in animal production. The best-characterized genetic control of disease resistance and immune response in animals is the one associated with the Major Histocompatibility Complex (MHC). The ovine lymphocyte antigenof DRB1 gene encodes cell surface glycoproteins that initiate immune responses by presenting processed antigenic peptides to...