abstract        background and objective: herpes simplex encephalitis is a life-threatening consequence of the central nervous system (cns) infection with herpes simplex virus (hsv). although it is a rare disease, mortality rates reach 70% in the absence of therapy and only a minority of individuals can return to normal function. the aim of this study was to determine possible correlation betwe...

background and objectives: pseudomonas aeruginosa (pa) is one of the most important causes of nosocomial infections and has an intrinsic resistance to many antibiotics. among all the resistance-nodulation-division (rnd) pumps of p. aeruginosa, mexab-oprm is the first efflux pump found to target multiple classes of antibiotics. this study was aimed to evaluate the expression level of genes expre...

background and objectives: a new real- time pcr protocol for the detection of trichomonas vaginalis in pooled urine samples has been optimized and validated. materials and methods: the amplification protocol, targeting a 2kb repeated gene in the t. vaginalis genome, was optimized by varying pcr parameters. as a reference method, a real-time pcr protocol targeting the beta-tubulin gene (y. versl...

Sensitive molecular techniques are needed for rapid detection of the R292K oseltamivir-resistant mutant of influenza A(H7/N9) virus strain to monitor its transmission and guide antiviral treatment. We developed a real-time reverse transcription PCR and single nucleotide polymorphism probes to differentiate this mutant strain in mixed virus populations in human specimens.

A bovine-specific cDNA microarray was used to characterize gene expression in the bovine rectoanal junction mucosa in response to Escherichia coli O157:H7 colonization, and results were confirmed using quantitative real-time PCR. The results showed involvement of cell processes including immune response, cell structure/dynamics, signal transduction, intercellular communication, and metabolism.

Stx bacteriophages in 68 samples of beef and salad were quantified by real-time quantitative PCR (qPCR). Stx phages from the samples were propagated in Escherichia coli C600, E. coli O157:H7, and Shigella strains and further quantified. Fifty percent of the samples carried infectious Stx phages that were isolated from plaques generated by lysis.