Stamenković, J., Radojković, I., Đorđević, A., Jovanović, O., Petrović, G., Stojanović, G.: Optimization of HPLC method for the isolation of Hypericum perforatum L. methanol extract. Biologica Nyssana, 4 (12), December 2013: 81-85. St. John's Wort (Hypericum perforatum L.) is one of the most studied plant species in the family Hypericaceae. The aim of this study was the identification of the co...

Background: Biotechnologic methods are common for secondary metabolites production from the plants and other sources in pharmaceutical sciences. Hairy root cell lines as the biotechnologic method have been used for in vitro production of major plant metabolites. Objective: In this study, hairy roots of Hypericum Perforatum have been prepared using the seeds and bio transformed by bacteria. Fin...

The effect of cadmium compounds containing selenium in different oxidation states such as Se(IV), Se(VI), and Se(–II) on production characteristics, shoot water content and chlorophyll content in the leaves as well as Cd and Se accumulation in plant organs of Hypericum perforatum plants was investigated. Complexes with nicotinamide (nia) of the type Cd(NCX)2(nia)2 where X = Se or S as well as C...

Hyperforin is an antibiotic, antibacterial, antiprotozoal, antiviral, anticancer, and immunomodulatory substance. It is possibly a new and potent tool for curative treatment of a broad array of infection diseases. Despite the medical usefulness of Hypericum perforatum L., which has been known since ancient times, its constituents were not well studied until the middle of the 20 century. Several...

Hypericum perforatum, is a plant which blooms between july and september at farms, borders of roads and woods, top of hills and grasslands, whose anti-inflammatory effects are shown in various studies. It is shown that cigarette smoke causes increase in inflammation mediators on lung. Due to this reason, we planned a study to examine the protective effects of Hypericum perforatum aganist smoke ...

Hypericum perforatum is a widely used medicinal plant known that it has high bioactive performance due to its special biochemical compounds in structure. The current study aimed determine the optimum processing parameters of extraction conditions constituents H. by ultrasound application and some performance. For this purpose, different variables namely sonication period (min), liquid/solid rat...

Hypericum perforatum L. (St. John’s wort) is a well-known medicinal plant that possesses secondary metabolites with beneficial pharmacological properties. However, improvement in the production of via genetic manipulation challenging task as H. remains recalcitrant to Agrobacterium tumefaciens-mediated transformation. Here, transcripts key genes involved several defence responses (secondary met...

The present study has been evaluated the neuroprotective effect of Hypericum perforatum extract on the reaction of astrocytes in mice brain treated with an intraperitoneal injection of 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) (20 mg/kg with 2 hr intervals). Treatment with Hypericum perforatum extract (HPE) resulted in an inhibition of monoamine oxidase-B (MAO-B) activity and reduced ...

background: st. john’s wort (hypericum perforatum l.) is an important medicinal plant that has been widely used for its antidepressant properties. objective: in this study morphological characteristics variation of populations of h. perforatum were investigated. methods: in this study, different morphological characteristics of 25 h. perforatum populations in iran’s natural habitats were evalua...

The crude methanolic extracts of six species of Hypericum [H. caprifoliatum Cham. & Schlecht., H. carinatum Griseb., H. connatum Lam., H. ternum A. St. Hil., H. myrianthum Cham. & Schlecht. and H. polyanthemum Klotzsch ex Reichardt] growing in southern Brazil were analyzed for antimicrobial activity against several microorganisms (bacteria and fungi). The most active plant was H. caprifoliatum,...