Lipid peroxidation represents a significant source of erythrocyte dysfunction and aging. Because the toxicity of lipid peroxidation appears to be in part due to aldehydic end products, we examined, in rat erythrocytes, the metabolism of 4-hydroxy-trans-2-nonenal (HNE), one of the most abundant and toxic lipid-derived aldehydes. Packed erythrocytes, 0.1 ml, completely metabolized 20 nmoles of HN...

The overexpression of either gamma-glutamylcysteine synthetase (gamma-ECS) or glutathione synthetase (GS) in Brassica juncea transgenics was shown previously to result in higher accumulation of glutathione (GSH) and phytochelatins (PCs), as well as enhanced Cd tolerance and accumulation. The present study was aimed at analyzing the effects of gamma-ECS or GS overexpression on tolerance to and a...

introduction: determination of best culture medium for fertilization and development of nmri mice. material and methods: development of fertilized mature oocytes of nmri mice was studied for five days in several culture media (ksom, t6, m16, czb, g1tm ver3 and g2tm ver3) following hormone therapy with pmsg and hcg. results: the rate of fertilization after 24 hours was 97.4% in ksom, 83.5% in t6...

background: this study quantified the effect of four popular culture media in a biodiesel production study on the qualitative and quantitative lipid content, dry biomass, and lipid productivity of nannochloropsis oculata. methods: culture of microalgae was done separately in walne, f/2, sato, and tmrl media. in the logarithmic and stationary growth phases, biomass production and lipid accumulat...

conclusions according to the results of the present study, it can be concluded that adipose derived mscs can be cultured in serum-free media with no change in their differentiating capacity. this finding gives us a hope for future cell therapy studies and trials with little concern about zoonotic infections or immunological reaction. results the isolated cells showed adherent spindle shaped mor...

Material obtained from the in vitro incubation of granulocytes from saline-induced peritoneal exudates of rabbits has been shown to produce inflammation and fever in rabbits. The supernatant material from cells incubated in saline has been termed granulocytic substance (GS) and is heat-labile. Its production is temperature dependent, occurring at 37 degrees C but not at 4 degrees C, requires vi...

Maturation of the mammalian oocyte is characterized in part by dissolution of the nuclear envelope, or germinal vesicle breakdown (GVB). By fluorescence microscopy after vital uptake of acridine orange (AO), redistribution and perinuclear accumulation of organelles corresponding to lysosomes occur before GVB in rat oocytes undergoing meiotic maturation in vitro. In follicle-enclosed oocytes exp...

introduction: cyclosporin a (cya) is a potent immunosuppressive drug widely used in transplant settings due to its specific inhibition of t–cell activation. several studies have reported the synergistic effect of cya with hematopoietic growth factor in colony culture of mice bone marrow. we studied the effect of il-3, il-6, scf,cya and nutrition medium ( condition medium - pha) on the developme...

We studied possible evolution of rotational period and magnetic field of the X-ray source RX J0720.4-3125 assuming this source to be an isolated neutron star accreting from interstellar medium. Magnetic field of the source is estimated to be 10 6 − 10 9 Gs (most probably ≈ 2 · 10 8 Gs), and it is difficult to explain the observable rotational period 8.38 s without invoking hypothesis of the mag...

large number of clinical cases in endodontics, however, cytotoxicity of such sealers must be tested before their validation as fi lling materials. the aim of this study was to evaluate the cytotoxic effect of a new copaiba oil-based root canal sealer (biosealer [bs]) on osteoblast-like osteo-1 cells. materials and methods: the experimental groups were formed according to the culture medium cond...