The present study aimed to investigate the anti-inflammatory effects of Baicalein on human osteoarthritic chondrocytes and its molecular mechanisms, explore related events that may occur. extracted were stimulated with interleukins. After administered different concentrations Baicalein, survival rate was observed. Apoptosis detected by apoptosis assay kit. effect NO production using Griess reag...

Nitrogen dioxide (NO2) is a major air pollutant with diverse impacts on human health and the environment. In urban areas, road traffic main emission source for NO2. Berlin, Germany, network of measurement stations operated by state, fulfilling monitoring requirements set European Union. To get more detailed overview spatial distribution NO2 concentrations in citizen science project allowed coll...

Environmental context Specific inhibitors of biological reactions in the nitrogen cycle can be used to determine origin reactive species; these species potentially degrade water quality or influence climate. However, interfere with methods for analysis stable isotope ratios and concentrations ammonium, nitrite nitrate. The effect this interference on several commonly was investigated. These fin...

The number of Alzheimer’s disease (AD) affected patients is increasing without any effective cure and the etiology remains to be understood. Inflammations, oxidative stress, Aβ, tauopathy are associated factors AD. Sodium butyrate (NaB) an HDAC inhibitor profoundly found neuroprotective. We have investigated neuroprotective effects NaB in SH-SY5Y cells stimulated with TNF-α/Aβ LPS-induced BV-2 ...

Standard calibration methods used to determine trace analytes usually yield significant deviations from the actual analyte value in the presence of interferents in the assay media. These deviations become of particular concern when the concentration of the analyte is low, and when the results are used to draw mechanistic or kinetic conclusions, for instance in enzyme structure-function studies....

Nitric oxide (NO) plays an important role in many inflammatory responses and is also involved in carcinogenesis. In the present study, we investigated the inhibitory effect of extracts from Humulus lupulus L. on both the production of NO and the expression of inducible NO synthase (iNOS) in mouse macrophage RAW 264.7 cells. The production of NO was induced by a combination of lipopolysaccharide...

To search for novel nitric oxide (NO) releasing anti-tumor agents, a series of novel furoxan/oridonin hybrids were designed and synthesized. Firstly, the nitrate/nitrite levels in the cell lysates were tested by a Griess assay and the results showed that these furoxan-based NO-releasing derivatives could produce high levels of NO in vitro. Then the anti-proliferative activity of these hybrids a...

An automated hydrodynamic sequential injection (HSI) system with spectrophotometric detection was developed. Thanks to the hydrodynamic injection principle, simple devices can be used for introducing reproducible microliter volumes of both sample and reagent into the flow channel to form stacked zones in a similar fashion to those in a sequential injection system. The zones were then pushed to ...

OBJECTIVE To investigate if salivary nitrate correlates to the daily psychological stress and anxiety in a group of human subjects. METHODS The convenient sample recruitment method was employed; data from seventy three subjects were analyzed. The Perceived Stress Scale (PSS) and Hamilton Anxiety Rating Scale (HAM-A) inventories were used to determine stress and anxiety scores respectively. Sa...