RNAs mediate many different processes that are central to cellular function. The ability to quantify or image RNAs in live cells is very useful in elucidating such functions of RNA. RNA aptamer-fluorogen systems have been increasingly used in labeling RNAs in live cells. Here, we use the malachite green aptamer (MGA), an RNA aptamer that can specifically bind to malachite green (MG) dye and ind...

Malachite green (MG), N-methylated diaminotriphenylmethane, is one of the most common dyes in textile industry and has also been used as an effective antifungal agent. However, due to its negative impact on the environment and carcinogenic effects to mammalian cells, there is a significant interest in developing microbial agents to degrade this type of recalcitrant molecules. Here, an Exiguobac...

The influence of low-molecular-weight organic acids (LMWOAs), such as malonic acid, ethylenediaminetetraacetic acid, and oxalic acid, on the Fenton degradation of organic pollutants was examined under visible irradiation (lambda > 450 nm). The Fenton degradation of malachite green in the dark was completely blocked in the presence of LMWOAs. It was found that either visible light irradiation or...

Malachite green has been used as an effective compound to control external fungal and protozoan infections of fish since 1933 but it has never been registered as a veterinary drug for use in food fish because of its potential carcinogenicity, mutagenicity and teratogenicity in mammals. The present paper reviews negative sideeffects of malachite green including its accumulation and persistence i...

In the present study, a marine strain of Trichoderma sp was used for degradation of a synthetic dye, malachite green. Individual and interaction effects of the physical and chemical factors that influenced the percentage of dye degradation were tested by response surface methodology. For optimization, enzyme production and dye degradation were assessed under different temperatures (5-40°C), pH ...

fungous infection to saprolegnia is the most important difficulties at the egg of acipenser persicus which is controlled by malachite green as an effective structure . because of the effects and adverse imposition on human and various fish, its use has been avoided in most of the world more than two decades approximately. this study was aimed to compare the antifungal effect of zinc oxid nano p...

A laser trapping-microspectroscopy technique combined with excitation energy transfer from a fluorescent cationic dye (Rhodamine B, RB) to a non-fluorescent cationic dye (Malachite Green, MG) was employed to study pH effects on the diffusion coefficients of MG (D(MG)) in single cation-exchange resin microparticles with the diameters of 16 μm. When RB-pre-adsorbed resin particles were soaked in ...

BACKGROUND The P27-P55 (lprG-Rv1410c) operon is crucial for the survival of Mycobacterium tuberculosis, the causative agent of human tuberculosis, during infection in mice. P55 encodes an efflux pump that has been shown to provide Mycobacterium smegmatis and Mycobacterium bovis BCG with resistance to several drugs, while P27 encodes a mannosylated glycoprotein previously described as an antigen...

MG and SRB aptamers, which are short RNA sequences originally selected only for binding to malachite green or sulforhodamine B, can greatly enhance the fluorescence of normally nonfluorescent triphenylmethane dyes. MG aptamer enhances the quantum yields of malachite green (MG) and a novel rigidized derivative, indolinyl malachite green (IMG) by >2000-fold. SRB aptamer brightens patent blue V an...

In this study lead oxide nanoparticles were synthesized by using ecofriendly and non-toxic Morus rubra extract. The obtained characterized XRD, SEM, EDX, BET FTIR techniques. Powder X-ray diffraction analysis revealed that PbO had crystallite structure of high purity. SEM survey shows the having in general uniform particle distribution sizes vary within range 22.4 to 29.2nm. As established EDX ...