Granulocytes, the most abundant types of leukocytes, are the first line of defense against pathogen invasion. However, the plasticity and diversity of granulocytes have been increasingly revealed, especially with regard to their versatile functions in orchestrating adaptive immune responses. A substantial body of recent evidence demonstrates that granulocytes can acquire the function as antigen...

Urinary tract infection (UTI) is a serious public health problem caused by variety of microorganisms, the most prevalent which are bacteria. The diversity immune system in urinary critical factor defense against infections. This research included 127 clinically suspected UTI patients that all treated with urine analysis and complete blood count test order to observe increased effects neutrophil...

The presence of several different autoantibodies (auto-AT) at the same time is a specific peculiarity “autoantibody profile” SLE (systemic lupus erythematosus). It known that induction auto-AT formation involves both nonspecific and antigen-specific immunoregulatory disorders. In apoptosis, primary changes in cell membrane composition or/and excretion intracellular compounds into intercellular ...

Hemocytin, a multidomain protein from Bombyx mori L. (Lepidoptera: Bombycidae), is an ortholog of von Willebrand factor and is expected to be a major mediator of hemocyte aggregation. Antiserum was generated against hemocytin, and immune staining of hemocytes, hemolymph, and nodules was performed. Hemocytin was observed in steady-state hemocytes but not in plasma, even after bacterial injection...

iv CHAPTER 1: INTRODUCTION 1 General Introduction 1 Objectives and Specific Aims 2 CHAPTER 2: LITERATURE REVIEW 6 CHAPTER 3: MATERIALS AND METHODS 15 Stx2 and rStxB2 15 Isolation of Porcine Granulocytes 16 Flow Cytometric Evaluation of Stx2 and rStxB2 Binding and Gb3/CD77 Expression by Porcine Granulocytes 17 Stx2 Binding to Porcine Granulocytes at 4°C 17 Flow Cytometric Analysis 19 Fluorescenc...

Unlike unanimous opinion on tissue factor (TF) expression in monocytes, the quest for TF presence in granulocytes has been going on for decades. To study the cell origin and track the blood-borne TF, we assessed TF activity and protein levels, knocked-down endogenous TF expression with small interfering RNA (siRNA), and overexpressed TF–yellow fluorescent protein (TF-YFP) fusion in immunologica...

We have characterized the erythrocytes, granulocytes, and platelets of 54 patients with paroxysmal nocturnal hemoglobinuria (PNH) with antibodies to glycosylphosphatidylinositol-anchored proteins (anti-CD55, anti-CD59, and anti-CD16) and flow cytometry to establish the usefulness of this technique in the diagnosis of this disorder. All patients demonstrated either completely (PNH III) or partia...

To clarify the pathologic significance of granulocytes exhibiting the paroxysmal nocturnal haemoglobinuria (PNH) phenotype in patients with aplastic anaemia (AA), we examined peripheral blood from 100 patients with AA for the presence of granulocytes deficient in glycosylphosphatidylinositol (GPI)-anchored proteins using a sensitive flow cytometric assay. A significant increase in the frequency...

BACKGROUND The phagocytosis of pathogens is essential for fighting infections. No assay is available, however, to measure both engulfment and degradation of bacteria under conditions similar to those in vivo. We sought to develop a flow cytometric assay to measure the engulfment and degradation of live bacteria by human blood monocytes and granulocytes. METHODS We generated enhanced green flu...

DNA content of isolated peripheral granulocytes and mononuclear cells was determined by flow cytometry and the results correlated with neutrophil chemotaxis. in 10 patients with secondary preleukemia or overt acute leukemia and cytogenetic defects of chromosome no. 7 in bone marrow cells. Nine patients had a hypodiploid clone with a missing chromosome no. 7. one lacked the long arm of a chromos...