Myelotoxic injury in the bone marrow (BM) as a consequence of total body irradiation (TBI) or granulocyte colonystimulating factor (G-CSF) mobilization results in the deposition of iC3b on BM stroma (stroma-iC3b). In the present study, we have examined how stromaiC3b interacts with hematopoietic progenitor cells (HPCs) and the role of complement (C) and complement receptor 3 (CR3) in BM injury/...

It was observed that interferon (IFN) prevents the down-regulation of the interleukin-3 receptor chain (IL-3R ), which spontaneously occurs during culture of human monocytes. The functionality of IL-3R was demonstrated by the fact that IL-3 rescued IFN–treated monocytes from apoptosis. Monocytes cultured in the presence of IFNand IL-3 acquire a dendritic morphology and express high levels of HL...

background: immunotoxins are comprised of both the cell targeting and the cell killing moieties. we previously established a new immunotoxin, i.e. shiga toxin granulocyte macrophage-colony stimulating factor (stxa1-gm-csf), comprises of catalytic domain of stx, as a killing moiety and gm-csf, as a cell targeting moiety. in this study, the ability of the immunotoxin to induce apoptosis and doubl...

oxidative metabolism and interleukin-8 production in human neutrophils exposed to azithromycin: effects of Streptococcus pneumoniae. J Antimicrob Chemother 2000;46:19–26. 30. Yamasawa H, Oshikawa K, Ohno S, et al. Macrolides inhibit epithelial cell-mediated neutrophil survival by modulating granulocyte macrophage colonystimulating factor release. Am J Respir Cell Mol Biol 2004;30:569–75. 31. Ho...

The high-affinity human granulocyte-macrophage colonystimulating factor (GM-CSF) receptor (GMR) consists of an alpha (GMRa) and a common beta (bc) subunit. The intracellular domain of bc has been extensively characterized and has been shown to be critical for the activation of both the JAK/STAT and MAP kinase pathways. The function of the intracellular domain of GMRa, however, is not as well ch...

Eosinophil chemotaxis and survival within tissues are key components in the development of tissue eosinophilia and subsequent effector responses. In this study, we demonstrate a novel mechanism of eosinophil autoregulation affecting migration and survival mediated through Notch signaling. We show for the first time that human blood eosinophils express Notch receptors and Notch ligands, expressi...

Cyclosporine (CsA)-induced gingival hyperplasia (CIGH) is a common side effect of CsA. A 54-year old woman was diagnosed as having severe aplastic anemia with trilineage cytopenia. Since she had no HLA-matched relatives, she was treated with immunosuppression including anti-lymphocyte globulin, methylprednisolone, recombinant human granulocyte colonystimulating factor and CsA (oral dose of 3.5 ...

Adoptive transfer of effector T cells has been used successfully to eliminate metastases in animal models. Because antitumor activity depends on the number of effector cells transferred, some human trials have used in vitro-repetitive activation and expansion techniques to increase cell number. We hypothesized that the prolonged culture period might contribute to the lack of human trial success...

Evidence is reviewed that indicates that an immune deficiency state may underlie patients with immune complex nephritis. Studies in several laboratories provide data that show defective immune function in patients with nephritis at the following loci: defective immunoglobulin production, impaired monocyte Fc and C3b receptor function, diminished complement biosynthesis, and decreased mesangial ...