Plant tissue culture is an alternative method of commercial propagation and is being used widely for the commercial propagation of a large number of plant species, including many medicinal plants. Somatic embryogenesis is a process by which asexual or somatic cells are induced to form embryos in culture. Somatic embryogenesis is a multi-step regeneration process starting with formation of pro-e...

Induction of somatic embryogenesis in absence of exogenous auxin can clarify some basic concepts of somatic embryogenesis.  Explants from leaf and petiole of Cucumber (Cucumis sativus) were used in this experiment for somatic embryogenesis using three different modified B5 basal media; boron and 2,4-D but no IAA as control (I), boron and IAA but no 2,4-D (II) and no boron, 2,4-D and IAA (III). ...

abstract in this research, the effects of ba with different concentrations (0, 0.5, 1, 1.5, 2, 2.5 and 3 mg/l) in combination with iba (0, 0.01 and 0.1 mg/l) on bud growth and shoot proliferation and tdz with different concentrations (0, 1, 2, 3 and 4 mg/l) in combination with 0 and 0.5 mg/l iba on somatic embryogenesis from different parts of immature cotyledons (proximal, median and distal) w...

background: ornithobilharziosis as one of the parasitic infections may give rise to serious economic problems in animal husbandry. the aim of the study was to prepare and compare the somatic and excretory-secretory (es) antigens of o. tur­kestanicum in gel diffusion test. methods: excretory-secretory (es) and somatic antigens of ornithobilharzia turkestanicum were prepared from collected worms ...

frequency of somaclonal variation in embryogenic calli and plants of olea europaea l cv.kroneiki derived from somatic embryogenesis were examined by randomly amplified polymorphic dna (rapd) analysis. radicle and cotyledon (proximal part) of mature zygotic embryo were cultured in omc medium supplemented with iba (5 mgl-1) and 2-ip (0.5 mgl-1). somatic embryogenesis was induced in calli of radic...

this study was carried out to investigate the morphological and anatomical aspects of somatic embryogenesis in date palm. lateral bud and shoot tip explants excised from young offshoots were cultured on ms medium with 2,4-d. somatic embryogenesis was in-duced by transferring the calli produced on the same medium without hormones. micro-tome sectioning of paraffin-embedded specimens was carried ...

mastitis is one of the most serious and costly diseases affecting dairy cattle production. in the present study, effects of a lactoferrin gene polymorphism (intron 6) on milk somatic cell count (scc) and subclinical mastitis was investigated in 121 holstein dairy cattle. two alleles of a and b and two genotypes of aa and ab were found in an ecori recognized single nucleotide polymorphism in int...