We evaluated cellular responses to polymer-treated gold nanorods, which were synthesized using the standard wet-chemistry method that utilizes hexadecyltrimethylammonium bromide (CTAB). The nanorod dispersions were coated with either polystyrene sulfonate (PSS) or polyethylene glycol (PEG). Two sizes of nanorods were tested, with optical responses peaking at 628 and 773 nm. The cells were from ...

Gold nanorods (AuNRs), rod-like gold nanoparticles, show strong plasmon bands in the near-infrared region, based on the longitudinal oscillation mode of surface plasmons. In this research, we have coated AuNRs with silica or poly (sodium 4-styrenesulfonate) (PSS) in order to evaluate the effects of surface modifications as a sensing probe material of AuNR. Multilayered assemblies of the PSS-coa...

Plasma membrane, coated pits, endosomes, and lysosomes were isolated from a mouse T lymphoma cell line using a density shift protocol in which these compartments were selectively loaded with gold conjugates. The plasma membrane was prepared after selective labeling for 1 h at 2 degrees C with gold-ricin and gave a yield of 40% according to enzymatic and antigenic markers. Endosomes were obtaine...

We have determined the membrane topography of the high-affinity IgE receptor, FcstraightepsilonRI, and its associated tyrosine kinases, Lyn and Syk, by immunogold labeling and transmission electron microscopic (TEM) analysis of membrane sheets prepared from RBL-2H3 mast cells. The method of Sanan and Anderson (Sanan, D.A., and R.G.W. Anderson. 1991. J. Histochem. Cytochem. 39:1017-1024) was mod...

A comparative study of the surface-enhanced Raman scattering (SERS) effect of the spherical gold nanoparticles (NPs) and highly uniform and oriented gold nanotriangles (NTs), anchored on indium tin oxide (ITO) coated conducting glass surfaces is reported. Contact and non-contact mode atomic force microscopic (AFM) analyses confirmed that all the triangles observed are equilateral and uniformly ...

Bioactive coatings are in high demand to increase the functions of cells for numerous medical devices. The objective of this in vitro study was to characterize osteoblast (bone-forming cell) adhesion on several potential orthopedic polymeric materials (specifically, polyetheretherketone, ultra-high molecular weight polyethylene, and polytetrafluoroethylene) coated with either titanium or gold u...

Upconversion of infrared light to visible light has important implications for bioimaging. However, the small absorption cross-section of rare earth dopants has limited the efficiency of these anti-Stokes nanomaterials. We present enhanced excitation absorption and single particle fluorescent emission of sodium yttrium fluoride, NaYF4: Yb, Er based upconverting nanoparticles coated with a gold ...

Thermal wetting can simply, selectively and reversibly join patchy particles into clusters (2D and 3D) and also colloidal crystals over the narrow temperature range of 1-2 °C. This is demonstrated with Janus particles (gold half-coated silica spheres) immersed in a binary mixture of water/2,6-lutidine, such that the relative strength of gold-gold bonding through hydrophobic interaction and sili...

Gold nanoparticles on Al2O3-coated gold films are presented as a chemically and thermally robust platform for molecular sensing. Single particle spectroscopy as a function of Al2O3 coating thickness shows reproducible gold nanoparticle scattering spectra in the range from 690 to 610 nm as the Al2O3 thickness increases from 0 to 3.4 nm. Numerical simulation of these structures indicates that sur...

In this article electrochemical determination of captopril at the surface of the platinum coated nanoporous gold film (PtNPGF) electrode is reported using the cyclic voltammetry and amperometry. For the preparation of PtNPGF, the surface of NPGF electrode was covered with Cu layer using underpotential electrochemical deposition (UPD).Afterward, the copper layer is replaced with platinum ions vi...