BACKGROUND The Fallopian tube undergoes well-recognized changes during the ovarian cycle. Ciliary beat frequency (CBF) increases during the secretory phase of the cycle. The stimulus is unknown, although CBF is known to be hormone responsive. At ovulation, follicular fluid is released into the peritoneal cavity and enters the Fallopian tube. We hypothesized that this fluid may provide the stimu...

The objective was to characterise the hormonal composition of follicular fluid from mares with distinct anovulatory-cystic follicles. Follicular fluid was aspirated from six mares that presented with cystic follicles and from pre-ovulatory follicles of five normal mares (controls). Differences in progesterone, oestradiol, testosterone, IGF-I and IGF binding were analysed using Fisher's exact te...

BACKGROUND Fatty acids are known to be critically important in multiple biological functions. Phospholipid fatty acids of follicular fluid, an important microenvironment for the development of oocytes, may contribute to the women's fertility and the efficacy of assisted reproduction techniques. The aim of this study was to investigate the effect of fatty acid composition of follicular fluid pho...

To evaluate the effect of follicular fluid on in vitro maturation, pig oocytes were cultured in the presence of hormones where 10% of fetal calf serum (FCS), 10% of follicular fluid from large follicles (l-pFF), 10% of follicular fluid from medium follicles (m-pFF) or no supplement were added. When oocytes where matured in medium containing the hormones the addition of different supplements did...

background: some evidences showed that the secretion of uterine tube vagina and follicular fluid (ff) affects x and y-chromosome populations. sperm selection with x or y chromosome can added to oocyte for gender desired. the isolation of x y sperm have done and all efforts in this field are done to make culture media similar to in vivo condition. the objective of this study was to find if the f...

The objective of the present study was to evaluate changes in equine follicular fluid insulin-like growth factor binding protein (IGFBP) proteolytic activity as well as steroid, IGF, and IGFBP concentrations during follicular development in the mare. Mares (n = 14) were classified as either in the follicular phase (n = 8) or luteal phase (n = 6). Follicles (n = 92) were categorized as small (6 ...

The biochemical components and factors like protein and enzyme components of follicular fluid like lipase, amylase and creatinine phosphokinase concentration show an increasing trend as the follicles mature, while some other components like Alpha-fetoprotein, Acid Phosphatase and Alkaline Phosphatase concentration decreases with increase in follicular size. Moreover, presence of amylase, lipase...

The main objective of the study was to asses whether the environmental cadmium exposure in the Czech Republic is high enough to be able to affect significantly the human reproduction. Cadmium levels were measured in the blood and follicular fluid of 220 women in in vitro fertilization (IVF) therapy, using atomic absorption spectrometry. The mean value of cadmium in venous blood was 0.85 ng x ml...

OBJECTIVE The objective of this study was to evaluate the relationship between oocyte yield, fertilization, and clinical pregnancy (CP), and anti-Mullerian hormone (AMH) level in serum and follicular fluid during in vitro fertilization treatment. METHODS Forty-four infertile women who underwent IVF treatment using multiagonist protocol were included in this study. Baseline level of AMH in ser...

Follicles from goat and sheep ovaries were characterized for their biochemical and hormonal parameters to investigate the effect of developmental stage of follicles on ovarian steroidogenesis. The follicles were isolated mechanically from follicular and luteal phase ovaries and divided in 6 morphologically different groups (small, medium and large follicular and small, medium and large luteal)....