STOREY, K. B. 1988. Mechanisms of glycolytic control during facultative anaerobiosis in a marine mollusc: tissue-specific analysis of glycogen phosphorylase and fructose-2,6-bisphosphate. Can. J. Zool. 66: 1767 177 1. Changes in the activity of glycogen phosphorylase and the content of fructose-2,6-bisphosphate (F-2,6-P2) were monitored in tissues of the whelk, Busycotypus canaliculatum, over a...

Al&met-The effects of epinephrine. norepinephriie, and isoproterenol on uterine phosphorylase were studied in intact, anesthetized rats. All three agents were found to increase uterine phosphorylase a activity when administered in large doses by intraperitoneal injection. Total phosphorylase activity was unaffected. The time course for the effects of epinephrine on uterine phosphorylase activit...

The presence of endogenous phosphorylase kinase and phosphorylase phosphatase in crude extracts of fat bodies from the cockroaches Nauphoeta cinerea and Periplaneta americana is demonstrated in vitro by activation/inactivation of glycogen phosphorylase under appropriate conditions. Fractionation of fat body extracts of both cockroach species on an anion-exchange medium results in the elution of...

1. The development of active and inactive phosphorylase was determined in rat liver during the perinatal period. No inactive form could be found in tissues from animals less than 19 days gestation or older than the fifth postnatal day. 2. The regulation of phosphorylase in organ cultures of foetal rat liver was examined. None of the agents examined [glucagon, insulin or dibutyryl cyclic AMP (6-...

Antibodies that are specific for the NHz-terminal region of rabbit muscle glycogen phosphorylase were isolated. Studies, using synthetic peptides representing different segments of the NHz-terminal region of muscle phosphorylase, indicated the antibodies are highly specific for the first 4 NHz-terminal residues of the enzyme. The molecular weight of the complex formed between dimeric phosphoryl...

Antibodies that are specific for the NHz-terminal region of rabbit muscle glycogen phosphorylase were isolated. Studies, using synthetic peptides representing different segments of the NHz-terminal region of muscle phosphorylase, indicated the antibodies are highly specific for the first 4 NHz-terminal residues of the enzyme. The molecular weight of the complex formed between dimeric phosphoryl...

The purpose of the study was to examine the activity of glycogen debranching enzyme, GDE, in porcine and bovine muscles differing in rate of contraction and oxidative capacity. The activity of GDE, the activity of phosphorylase, total glucose content, lactate content and pH were measured from meat samples taken 35min post-mortem and ultimate pH 24 or 48h post-mortem. Both GDE and phosphorylase ...

Experimental animal models of diabetes induced either by alloxan or streptozotocin have been used to study aspects of the pathophysiology of this disease. The purpose of this study was to examine the metabolism of glycogen in the submandibular and parotid salivary glands of diabetic rats. Diabetes was induced by an intraperitoneal injection of streptozotocin. Eight weeks after the induction of ...

Phosphorylase kinase (PhK) coordinates hormonal and neuronal signals to initiate the breakdown of glycogen. The enzyme catalyzes the phosphorylation of inactive glycogen phosphorylase b (GPb), resulting in the formation of active glycogen phosphorylase a. We present a 9.9 angstroms resolution structure of PhK heterotetramer (alphabetagammadelta)4 determined by cryo-electron microscopy single-pa...

A series of morpholine substituted amino acids (phenylalanine, leucine, lysine and glutamic acid) was synthesized. A fragment-based screening approach was then used to evaluate a series of small heterocycles, including morpholine, oxazoline, dihydro-1,3-oxazine, tetrahydro-1,3-oxazepine, thiazoline, tetrahydro-1,3-pyrimidine, tetrahydro-1,3-diazepine and hexahydro-1H-benzimidazole, as potential...