The sustainable deployment of resistant crop varieties is a critical issue for the implementation of biotechnology in crop pest management. Feeding, biomass accumulation, and mortality were evaluated for susceptible, insecticide-resistant, and Bacillus thuringiensis (Bt) Cry 3A-selected Colorado potato beetle ( Leptinotarsa decemlineata Say) (Coleoptera, Chrysomelidae) larvae fed on: cultivated...

Background The dermatophytes belong to one of the main groups of pathogenic fungus, characterized by the use of the host’s keratin for its nutrition, which are the most common cause of fungal infection in the world, affecting millions of individuals annually, causing a huge economic impact [1]. Therefore, there was an increase in the search for new antifungal agents from natural sources, becaus...

Variation in resource inputs to plants may trigger bottom-up effects on herbivorous insects. We examined the effects of water input: optimal water vs. limited water; water salinity: with vs. without addition of 100 mM NaCl; and their interactions on tomato plants (Solanum lycopersicum), and consequently, the bottom-up effects on the tomato leaf miner, Tuta absoluta (Meytick) (Lepidoptera: Gelec...

Eggplant is one of the most important vegetable crops known for its nutritive benefits due to abundance various bioactive compounds, which include proteins, vitamins, minerals, carbohydrates, phenolics, and dry matter content. In addition, eggplant has significant pharmaceutical properties that have been recently recognized. produces secondary metabolites, including glycoalkaloids, antioxidant ...

Potatoes (Solanum tuberosum) contain α-solanine and α-chaconine, two well-known toxic steroidal glycoalkaloids (SGAs). Sprouts and green tubers accumulate especially high levels of SGAs. Although SGAs were proposed to be biosynthesized from cholesterol, the biosynthetic pathway for plant cholesterol is poorly understood. Here, we identify sterol side chain reductase 2 (SSR2) from potato as a ke...

The production of low-molecular-weight antimicrobial molecules within plants is one component of defense against pathogens. Among them, preformed antimicrobial compounds (phytoanticipins) are the first biochemical barriers against pathogens. Saponins (a group of phytoanticipins) are present constitutively in plants and play important roles in plant defense. A variety of biological roles have be...

Steroidal glycoalkaloids (SGAs) existing in most potato tissues are toxic to humans when the fresh weight is over 200 mg kg?1, and high SGAs content would also damage quality of tubers. Sterol side chain reductase 2 (StSSR2) a key enzyme biosynthetic potatoes. To reduce concentration tetraploid cultivated (Solanum tuberosum L.), gene StSSR2 was edited by CRISPR/Cas9 system. A total number 64 mu...

A quantitative human dietary risk assessment was conducted using the glycoalkaloid concentrations measured from tubers of plants defoliated by Colorado potato beetles and undefoliated (control). There was a significantly greater production of glycoalkaloids for defoliated plants compared to control plants for both skin and inner tissue of tubers. The dietary risk posed to different human subgro...