The effect of the alpha-glucosidase inhibitor acarbose on retinal capillary basement membrane thickening was examined in the spontaneously diabetic BB/W-rat. Four months of diabetes resulted in significant thickening of the basement membranes of both the superficial and deep capillary nets of the retina. This characteristic change of the retinal microvasculature in diabetes was completely preve...

The degradation of cyanogenic glycosides was studied in spontaneously fermenting cassava root pulp and in fresh pulp samples pretreated to prevent either endogenous beta-glycosidase activity, fermentation, or both. The rate of disappearance of the glycosides, as measured by hydrocyanic acid (HCN) production in situ, in membrane-sterilised media or in samples containing 1% sodium iodoacetate, wa...

The essential biological roles played by glycosidases, coupled to the diverse therapeutic benefits of pharmacologically targeting these enzymes, provide considerable motivation for the development of new inhibitor classes. Cyclophellitol epoxides and aziridines are recently established covalent glycosidase inactivators. Inspired by the application of cyclic sulfates as electrophilic equivalents...

1756-4646/$ see front matter 2013 Elsevi http://dx.doi.org/10.1016/j.jff.2013.04.015 * Corresponding authors. Tel.: +82 2 970 6740 E-mail addresses: [email protected] Mulberry leaf extract was investigated as a potent alpha-glucosidase inhibitor, especially for individuals with prediabetes. We evaluated the effect of the ingestion of mulberry leaf aqueous extract (MLAE) on postprandial glu...

Feeding experiments with Actinoplanes sp. SN223/29 showed that 3-amino-5-hydroxy-[7-13C]benzoic acid is not incorporated into acarbose (I). The valienamine moiety of I is thus not derived in the same way, from the shikimate pathway, as the m-C7N units in the ansamycin, mitomycin and ansamitocin antibiotics. Feeding experiments with [U-13C3]-glycerol followed by analysis of I by multiple quantum...

In this double-blind, cross-over, placebo-controlled, randomized study, possible extraintestinal effects of miglitol, an absorbable alpha-glucosidase inhibitor, were investigated. Sixteen healthy male volunteers underwent two 75 g oral glucose tolerance tests with concomitant administration of miglitol or placebo. Peak and post-peak areas under the curve values for blood glucose, serum insulin ...

The Dayak tribe in Kalimantan has long used bawang dayak as a traditional cure for various degenerative diseases. Previous research showed that bulb an active compound can inhibit the enzyme alpha-glucosidase, known alpha-glucosidase inhibitor (AGI). Compounds have such ability potential controlling blood glucose level diabetes mellitus. This aims to study effective dosage of aqueous extract an...

A wide range of proteinaceous inhibitors are present in plants to protect themselves from hydrolytic enzymes. In this study, turmerin, a water-soluble peptide in turmeric rhizomes, was evaluated for its inhibitory potential against glucosidase and its antioxidant (AO) capacity. Turmerin inhibited α-amylase and α-glucosidase activities with IC₅₀ values 31 and 192 µg mL⁻¹, respectively. Under the...

Aqueous extracts of four seaweeds collected from Gulf of Mannar coastal waters were tested for α-amylase and α-glucosidase inhibition properties. The aqueous extracts of seaweeds in the order of Gracilaria edulis, Sargassum polycystum, Ulva lactuca and Gracilaria corticata showed significant inhibitory activity against α-amylase and α-glucosidase enzymes. G. edulis was found to be a potent inhi...

This study aimed to isolate a potent antiglucosidase and antioxidant fraction from Stenochlaena palustris. Extraction was performed with hexane, chloroform, ethyl acetate, methanol, and water. Antiglucosidase, 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging and ferric reducing antioxidant power (FRAP) assays found methanol extract (ME) to be the most active. Water fraction (WF) of ME was a stro...