The biocontrol activity of Aureobasidium pullulans on decay of apple fruit caused by Botrytis cinerea and Penicillium expansum, and its ability to induce biochemical defense responses in apple tissue, were investigated. In apple wounds, A. pullulans multiplied rapidly and controlled decay caused by either B. cinerea or P. expansum. At the end of the storage period, A. pullulans reduced the inci...

J. Anim. Sci. Vol. 88, E-Suppl. 2/J. Dairy Sci. Vol. 93, E-Suppl. 1/Poult. Sci. Vol. 89, E-Suppl. 1 T203 Influences of four kinds of exogenous enzymes on performance, jejunal digesta viscosity and litter moisture of broilers fed wheat-based diet. H. Shirzadi*, H. Moravej, and M. Shivazad, University of Tehran, Karaj, Tehran, Iran. The intention of this work was to determine the effects of 4 enz...

The distribution of chitin in Saccharomyces cervisiae primary septa and cell walls was studied with three methods: electron microscopy of colloidal gold particles coated either with wheat germ agglutinin or with one of two different chitinases, fluorescence microscopy with fluorescein isothiocyanate derivatives of the same markers, and enzymatic treatments of [14C]glucosamine-labeled cells. The...

Gliocladium virens is a common saprophytic fungus that is mycoparasitic on a large number of fungi. Responses of G. virens toward its environment were examined by monitoring the presence of extracellular proteins in culture fluid during time course experiments. Culture fluid of G. virens grown on glucose, washed cell walls of Rhizoctonia solani (one of its hosts), olive oil, or chitin contained...

Morphology changes in bacterial cellulose produced by Acetobacter xylinum ATCC23769 were ob­ served in the presence of /3 -glucodisaccharides such as gentiobiose and cellobiose. Endo-/3 -1,4-glucanase ac­ tivity in culture broth was higher than that in the absence of those sugars. So we have investigated the prop­ erties of endo-/3 -1,4-glucanase (AEG) produced by this bacterium. This enzyme co...

Systemic acquired resistance (SAR) in Carica papaya L. is induced by benzothiadiazole (BTH). The response is manifested by increased tolerance to infection by the virulent pathogen Phytophthora palmivora, by increased b-1,3-glucanase and chitinase activities, and by increased accumulation of a PR1 mRNA. Infection of untreated papaya by P. palmivora also induced b-1,3-glucanase and chitinase act...

An enzyme that has both beta-1,4-glucanase and chitosanase activities was found in the culture medium of the soil bacterium Lysobacter sp. IB-9374, a high lysyl endopeptidase-producing strain. The enzyme was purified to homogeneity from the culture filtrate using five purification steps and designated Cel8A. The purified Cel8A had a molecular mass of 41 kDa, as estimated by sodium dodecyl sulfa...

In this study, effects of single and combined applications biocontrol agents; Trichoderma spp. (T. atroviride, T. virens) plant growth promoting rhizobacteria (Pseudomonas koreensis, Bacillus subtilis) on growth, wilt disease severity caused by Verticillium dahliae defence-related enzymes (peroxidase, polyphenol oxidase, phenylalanine ammonium lyase β-1,3 glucanase) eggplant, were investigated....

BACKGROUND Benzoic acid is one of the most useful aromatic compounds. Despite its versatility and simple structure, benzoic acid production using microbes has not been reported previously. Streptomyces are aerobic, Gram-positive, mycelia-forming soil bacteria, and are known to produce various kinds of antibiotics composed of many aromatic residues. S. maritimus possess a complex amino acid modi...

In a previous paper, we reported the first stopped-flow experiments on a Bacillus licheniformis 1,3-1,4-beta-glucanase [Abel, Planas and Christensen (2001) Biochem. J. 357, 195-202]. It was shown that the pre-steady-state kinetics of the 1,3-1,4-beta-glucanase using the substrate 4-methylumbelliferyl 3-O-beta-cellobiosyl-beta-D-glucoside may be explained by a reaction scheme involving an induce...