T FIAT VITAMIN B12 IN SERUM is l)ound to protein14 has been known since shortly after the isolation of the vitamin. Endogenous vitamin B12 (which may be mainly methyl-Bi2) is bound primarily to an a1-globulin 2.6-10 hut vitamin B12 added to serum in vitro hinds primarily to -globuun (presumably because B12-binding a-globulin is normally almost saturated with endogenous vitamin B12).61#{176} hal...

Various technics for concentrating cerebrospinal fluid (CSF) prior to electrophoretic separation of the protein fractions were tested. Vacuum ultrafiltration through a collodion sac proved to be the most reliable and convenient method. The proteins in the concentrate were separated by cellulose acetate electrophoresis and quantitated densitometrically. The mean values for the various protein fr...

Five failures of postexposure treatment of rabies in small children with multiple severe bites on the face and head are discussed. All had received rabies immune globulin and a potent tissue-culture vaccine. However, not all wounds had been infiltrated with immune globulin. Surgical closure prior to wound injection with immune globulin was performed in three cases. Another patient had wounds su...

T FIAT VITAMIN B12 IN SERUM is l)ound to protein14 has been known since shortly after the isolation of the vitamin. Endogenous vitamin B12 (which may be mainly methyl-Bi2) is bound primarily to an a1-globulin 2.6-10 hut vitamin B12 added to serum in vitro hinds primarily to -globuun (presumably because B12-binding a-globulin is normally almost saturated with endogenous vitamin B12).61#{176} hal...

T FIAT VITAMIN B12 IN SERUM is l)ound to protein14 has been known since shortly after the isolation of the vitamin. Endogenous vitamin B12 (which may be mainly methyl-Bi2) is bound primarily to an a1-globulin 2.6-10 hut vitamin B12 added to serum in vitro hinds primarily to -globuun (presumably because B12-binding a-globulin is normally almost saturated with endogenous vitamin B12).61#{176} hal...

Protein status of 194 pregnant women and their newborn at a public and a private maternity hospital in Tehran was studied. Blood samples were taken from all women before delivery and from cords at delivery of the placenta. The following determinations were made: total serum protein, protein fractions, creatinine and urea. In the private group, significantly higher maternal mean values were...

Normal 7S human gamma-globulin was found to contain two fundamental antigenic groups of molecules. The group 1 molecules of normal gammaglobulin correspond antigenically to group 1 multiple myeloma proteins and Bence Jones proteins; and group 2 molecules of normal gamma-globulin correspond antigenically to group 2 multiple myeloma proteins and Bence-Jones proteins. Among pooled human Fr II and ...

Intravenous immunization of New Zealand red rabbits with streptococcal group-specific bacterial vaccines yielded sera which possessed markedly elevated gamma-globulin. The sera of one rabbit immunized with Group A-variant vaccine possessed 55 mg/ml of gamma-globulin. The bulk of this gamma-globulin, identified as gamma(G)-globulin, was homogeneous by zone electrophoresis and of specificity dire...

Under a copper-deficient regimen, pancreatic cells in the adult rat can be found to undergo differentiation into hepatocytes. Pancreatic hepatocytes induced in male and female rats were examined for the expression of the androgen-inducible hepatic protein, alpha 2u-globulin. Alpha 2u-Globulin protein was demonstrable by immunoperoxidase method in all the pancreatic hepatocytes of male rats. Nor...

Gamma globulin formation in vitro by various tissues was studied using the incorporation of C(14)-L-lysine into a protein precipitable by a specific anti rabbit gamma globulin serum prepared in a sheep. It was demonstrated that the rate of gamma globulin formation in similar numbers of spleen cells is much higher if taken from an immunized rabbit at the height of antibody formation than that in...