This paper outlines a straightforward, fast, and low-cost method to fabricate polydimethylsiloxane (PDMS) chips. Termed sandwich bonding (SWB), this method requires only a laboratory oven. Initially, SWB relies on the reversible bonding of a coverslip over PDMS channels. The coverslip is smaller than the substrate, leaving a border around the substrate exposed. Subsequently, a liquid composed o...

BACKGROUND The cytosol of amoeba cells controls the membrane deformation during their motion in vivo. To investigate such ability of the cytosol of amoeba cell, Dictyostelium discoideum (Dictyostelium), in vitro, we used lipids extracted from Dictyostelium and commercially available phospholipids, and prepared substrate-supported lipid membrane patches on the micrometer scale by spin coating. ...

We report the fabrication and the optical characterization of a hybrid tunable integrated optical filter. It consists of a diffused ion-exchanged channel waveguide on a borosilicate glass substrate with a cover of the same glass to form a gap filled with a holographic grating. The grating morphology, called POLICRYPS (POlymer LIquid CRYstal Polymer Slices), is made of alternating stripes of pol...

Attachment of M. pneumoniae to glass was quantitated in an experimental system enabling the settling down of [3H]palmitic acid-labeled cells onto glass cover slips. Attachment of mycoplasmas suspended in buffer increased with temperature, decreased with higher ionic strength, and showed a maximum at about pH 5.5. The findings suggest a participation of ionic bonds in the attachment process. Try...

Headlamps on vehicles are primarily responsable for illuminating the traffic space during periods of low visibility, such as night or precipitation. In addition, headlamps are also considered as a cosmetic part, playing an important role in the car styling. Because of this, carmakers request a great precision for surface defect detection in all the headlamp components. One of these components i...

In order to perform an experiment into optical phase-only correlation it was necessary to fabricate an SLM to the highest standards possible. Chapter five has provided a background to the electronic operation of the 16×16 SLM and the liquid crystal light modulation mechanism used in this project. Actual fabrication of a working spatial light modulator is an extremely delicate process requiring ...

fluid. Many different fluids have been employed for this purpose, the one we prefer being Vibert's fluid (Common salt 2 grammes, Mercury Perchloride 0.5 gramme, Distilled water 100 c.c.). The clot is soaked in a few drops of Vibert's fluid in a watch glass for twenty minutes, care being taken to avoid evaporation. Then a portion of the softened clot is gently teased and transferred to a slide b...

The optimal path cover problem is to find a minimum number of vertex disjoint paths which together cover all the vertices of the graph. Finding an optimal path cover for an arbitrary graph is known to be NP-complete [3]. However, polynornial-time algorithms exist for trees [4], cacti [4] and for interval graphs [9]. The solution presented in [2] For circular-arc graphs is known to be wrong. In ...