We present a computationally efficient method for analyzing H&E stained digital pathology slides with the objective of discriminating diagnostically relevant vs. irrelevant regions. Such technology is useful for several applications: (1) It can speed up computer aided diagnosis (CAD) for histopathology based cancer detection and grading by an order of magnitude through a triage-like preprocessi...

Pathology has become increasingly more reliant on digital imaging as a means for viewing, sharing, and archiving slides, and as an essential rst step for the application of advanced image analysis to support cancer diagnostics. In H&E stained tissue, cell nuclei are especially prominent, and their shapes, staining attributes, and distributions within the tissue serve as important diagnostic and...

An improved silver carbonate impregnation method for marine ciliates is described. Compared to conventional methods, the new method of fixing and staining is easy to learn and yields easily reproducible results. Structures that are impregnated with silver carbonate such as nuclear apparatus, infraciliature as well as cortical and cytoplasmic microtubules and even contractile vacuole pore are st...

Gram stains of 87 different clinical samples were prepared by the laboratory's conventional methods (automated or manual) and by a new single-slide-type automated staining instrument, GG&B AGS-1000. Gram stains from either heat- or methanol-fixed slides stained with the new instrument were easy to interpret, and results were essentially the same as those from the methanol-fixed slides prepared ...

The examination of a well-stained film of either clinical material or organisms grown in laboratory media constitutes an important part of most investigations in hospital microbiology laboratories. The staining of large numbers of microscope slides, usually by Gram's method, is however timeconsuming, and a number of reports have been made on methods mechanising this procedure. Cremer (1968) and...

BACKGROUND Significant variation is reported in the diagnosis of HPV-associated AIN. We previously observed that band-like positivity for p16 in >90% of contiguous cells coupled with Ki67 positivity in >50% of lesional cells is strongly associated with high grade AIN. This study was undertaken to determine if addition of p16 and Ki67 immunostaining would reduce inter- and intraobserver variabil...

BACKGROUND Hirschsprung disease is a complex genetic disorder of the enteric nervous system (ENS), often called congenital aganglionic megacolon and characterized by the absence of enteric neurons along a variable length of the intestine. The definitive diagnosis of Hirschsprung disease relies on histologic and/or histochemical staining of sections from suction rectal biopsies. Calretinin immun...

Objectives: The aim of this study was to evaluate of fetal chromosome analysis in amniocentesis and fetal blood sampling cases. Method: We analysis of 649 cases between January 2007March 2009 in Dicle University Medical Faculty Department of Genetic. The study was included 552 amniocentesis and 97 fetal blood sampling cases. Lymphocyte culture prepared in duplicate and totally ten slides were p...

The present External Quality Assessment (EQA) assessed microscopy of blood parasites among diagnostic laboratories in the Democratic Republic of the Congo. The EQA addressed 445 participants in 10/11 provinces (October 2013-April 2014). Participants were sent a panel of five slides and asked to return a routinely stained slide which was assessed for quality of preparation and staining. Response...

Ultrastructural studies of cytoplasmic droplets of the spermatozoa of bulls, rams, rabbits, rats (Bloom & Nicander, 1961) and bats (Fawcett & Ito, 1965) revealed only numerous membranous vesicles and tubules in the matrix of the droplets. These authors agreed that the membranous elements were probably of endoplasmic reticulum and/or Golgi complex origin. The simplicity of the ultrastructure and...