OBJECTIVE There is a lack of information about the genotype frequencies of IL-6 -174G/C and -572G/C polymorphisms in Mexicans with rheumatoid arthritis (RA). Therefore, the aim of this study was to evaluate the association of the IL-6 -174G/C and -572G/C polymorphisms in Mexican mestizo with RA. METHODS We included 137 patients with RA and 102 healthy controls. Patients were assessed for clin...

The nucleotide sequence of hepatitis C virus (HCV) genotype 6 found mostly in south China and south-east Asia, displays profound genetic diversity. The aim of this study to determine the genetic variability of HCV genotype 6 (HCV-6) in Thailand and locate the subtype distribution of genotype 6 in various geographic areas. Four hundred nineteen anti-HCV positive serum samples were collected from...

Objective: Assessment of relevance of IL-6 (-174G>C, -596G>A) genes polymorphisms in the etiology of preterm delivery (PTD). Material and methods: Study group: 150 women with PTD (22+0 36 + 6 gw.); controls: 150 women who delivered > 37 gw. Genetical analysis was performed by PCR/RLFP method. Results: In PTD group -596GA genotype (48.6 vs. 42.7%) and -596AA genotype (24.7 vs. 21.3%) occurred mo...

BACKGROUND The PCR-Invader assay is a robust, homogeneous assay that has been shown to be highly sensitive and specific in genotyping single-nucleotide polymorphism (SNP) markers. In this study, we introduce two changes to improve the assay: (a) we streamline the PCR-Invader method by assaying both alleles for each SNP in one reaction; and (b) we reduce the cost of the method by adopting fluore...

It is well known that genotyping errors lead to loss of power in gene-mapping studies and underestimation of the strength of correlations between trait- and marker-locus genotypes. In two-point linkage analysis, these errors can be absorbed in an inflated recombination-fraction estimate, leaving the test statistic quite robust. In multipoint analysis, however, genotyping errors can easily resul...

Pyruvate dehydrogenase (PDH) plays a key role in the regulation of skeletal muscle substrate utilization. IL-6 is produced in skeletal muscle during exercise in a duration dependent manner and has been reported to increase whole body fatty acid oxidation, muscle glucose uptake and decrease PDHa activity in skeletal muscle of fed mice. The aim of the present study was to examine whether muscle I...

An assessment of hybrids medium staple cotton by fiber quality in the Tashkent, Fergana and Kashkadarya regions Republic Uzbekistan has been carried out. Four hybrid combinations different genetic origins were tested. The analysis influence genotype environment on manifestation qualitative parameters (length, specific breaking load, microneir) was High values found with participation wild speci...

Hepatitis C viruses (HCVs) display a high level of sequence diversity and are currently divided into six genotypes. A line probe assay (LiPA), which targets the 5' untranslated region (5'UTR) of the HCV genome, is widely used for genotyping. However, this assay cannot distinguish many genotype 6 subtypes from genotype 1 due to high sequence similarity in the 5'UTR. We investigated the accuracy ...

The study aimed to evaluate the genetic diversity of eight brinjal (Solanum melongena L.) genotypes including wild species and develop a molecular profile using 14 SSR primers. value similarity matrix ranged from 0.12–0.87. Dendrogram image is revealing relationship among brinjal. super cluster divided into two clusters A B at Jaccard’s coefficient 0.31. Cluster again divide sub A1 A2 0.45. con...