Background and Aims: Borna disease virus (BDV) is well known as a neurotropic virus, however, its role in human neurological diseases such as schizophrenia and bipolar disorder is still unclear. In this study, we aimed to investigate the BDV genome in such patients in Golestan province, North of Iran. Materials and Methods: RNA was extracted from peripheral blood mononuclear cells (PBMCs) from...

The recent development of homologous recombination-mediated gene targeting (GT) techniques has made it easy to modify nucleotide sequences in plant genomes. Backcross breeding following GT provides transgene-free lines with high probability. However, owing the possibility unintentional transgene introduction during genetic transformation, analytical methods may be necessary reliably and compreh...

Linkage disequilibrium (LD) is bases of genomic selection, genomic marker imputation, marker assisted selection (MAS), quantitative trait loci (QTL) mapping, parentage testing and whole genome association studies. The Particular alleles at closed loci have a tendency to be co-inherited. In linked loci this pattern leads to association between alleles in population which is known as LD. Two metr...

Background and Aims: Crimean-Congo hemorrhagic fever (CCHF) is a fatal viral disease that occurs in approximately 30 countries. It has the most extensive geographic range among the tick-borne viruses that affect human health. CCHF viruses have a tripartite RNA genome consisting of large (L), medium (M) and small (S) segments. This study was undertaken to determine the genetic relationship of CC...

Objective(s): Oncolytic Herpes simplex virus type 1 (HSV-1) has emerged as a promising strategy for cancer therapy. However, development of novel oncolytic mutants has remained a major challenge owing to low efficiency of conventional genome editing methods. Recently, CRISPR-Cas9 has revolutionized genome editing.Materials and Methods: I...

Abstract Powdery mildew disease caused by Oidium arachidis poses a threat to peanut production in Africa. Loss of function mutants specific Mlo (Mildew Locus O) genes have provided broad-spectrum and durable resistance against pathogen many crop species. Since there is huge potential utilize susceptibility gene-mediated improvement, genome-wide mining required for further researches. However, t...

background: causing site direct mutation can be one of the efficient methods to evaluate the characteristics and properties of various genes. brucellosis is the most common zoonotic infectious disease that would cause great economic losses. thus, recognition of pathogenic and immunogenic factors in the genus brucella can lead to control this health problem. objectives: considering the importanc...

background: dna immunization with plasmid dna encoding bacterial, viral, parasitic, and tumor antigens has been reported to trigger protective immunity. the use of plasmid dna vaccinations against many diseases has produced promising results in animal and human clinical trials; however, safety concerns about the use of dna vaccines exist, such as the possibility of integration into the host gen...