BACKGROUND Bovine neonatal pancytopenia (BNP) is a disease syndrome in newborn calves of up to four weeks of age, first observed in southern Germany in 2006. By now, cases have been reported in several countries around the globe. Many affected calves die within days due to multiple haemorrhages, thrombocytopenia, leukocytopenia and bone marrow depletion. A certain vaccine directed against Bovin...

Hepatitis delta virus (HDV) is composed of four specific components. The first component is envelope protein which contains hepatitis B surface antigens. The second and third components are nucleocapsid proteins, referred to as small and large hepatitis delta antigens (HDAgs). The final component is a single-stranded circular RNA molecule known as the viral genome. In order to study the mechani...

Glycosphingolipids of rhesus monkey kidney (MK), bovine kidney (MDBK), and two lines of hamster kidney (BHK21-F and Hak) cells have been compared with those of parainfluenza (SV5) virions grow in these cells. There are qualitative and quantitative differences in the neutral glycolipids and gangliosides found in the various cells. Cells with a high neutral glycolipid content (MK and MDBK) contai...

objective: β-thalassemia is caused by absence or reduction of β-globin chain synthesis. one of the effective therapeutic methods for this disease can be gene therapy by viral vectors. the capacity of lentiviral vectors is approximately 8 kb, we designed a 6 kb construct containing mini lcr and β-globin gene instead of lcr region. the aim of this study is to make a recombinant lentiviruses conta...

OBJECTIVE To construct a lentiviral vector expressing HIV-1 Tat and identify its expression in 293T cells. METHODS The gene fragment of HIV-1 Tat101 was subcloned to lentiviral transfer vector pHAGE-CMV-MCS-IZsGreen, which was named pHAGE-Tat. Then the constructed pHAGE-Tat was used to co-transfect the packing 293T cells, together with the packaging plasmids pMD2.G and psPAX2. The packaged vi...

The paramyxoviruses measles (subacute sclerosing panencephalitis, SSPE) virus and canine distemper virus (CDV) cause an impairment of the catecholamine-induced beta-adrenergic receptor-dependent cAMP generation in persistently infected C6 rat glioma cells. In C6 cells persistently infected with CDV the number of receptors is greatly reduced. Hirata and Axelrod have shown that the number of beta...

sulphur mustard (sm) is an alkylating agent that was first used as a chemical warfare agent during the first world war in 1917. sm is readily absorbed from the skin, respiratory and gastrointestinal tract and is distributed to several organs. sm may act rapidly and persistently upon dna replicating mechanism of the individual cells during mitosis particularly in the hematopoietic system. of 233...