نتایج جستجو برای: gene library

تعداد نتایج: 1247519  

The purpose of this study was comparing Information Retrieval Capabilities in Web-based Library Software of Payam, with Voyager and ALEPH. A checklist designed and included six main trait for evaluation and comparing 73 scales. Data collected by experts' observing of the software's OPAC. Data analyzed by the descriptive statistics methods. Findings shows the preferences in search capabilities i...

Journal: :DNA research : an international journal for rapid publication of reports on genes and genomes 1996
M Ohira A Ootsuyama E Suzuki H Ichikawa N Seki T Nagase N Nomura M Ohki

The Down syndrome (DS) region on chromosome 21, which is responsible for the DS main features, has been defined by analysis of DS patients with partial trisomy 21. Within the DS region, we constructed a 1.6-Mb P1 contig map previously. To isolate gene fragments from the 1.6-Mb region, we performed direct cDNA library screening and exon trapping using the P1 clones and a human fetal brain cDNA l...

Journal: :Journal of experimental zoology. Part B, Molecular and developmental evolution 2006
Jing Luo Michael Lang Walter Salzburger Nicol Siegel Kai N Stölting Axel Meyer

A goldfish (Carassius auratus auratus) bacterial artificial chromosome genomic library (BAC library) was constructed from one aquarium-bred male specimen (tetraploid, 4n=100, genome size=3.52 pg/cell). The library consists of 128,352 positive clones with an average insert size of 150.4 kb, covering the genome 11-fold. All clones were spotted onto nylon filters and thus are available for screeni...

Journal: :The Journal of clinical investigation 1986
A T Look S C Peiper M B Rebentisch R A Ashmun M F Roussel R S Lemons M M Le Beau C M Rubin C J Sherr

DNA from a tertiary mouse cell transformant containing amplified human sequences encoding a human myeloid membrane glycoprotein, gp150, was used to construct a bacteriophage lambda library. A single recombinant phage containing 12 kilobases (kb) of human DNA was isolated, and molecular subclones were then used to isolate the complete gp150 gene from a human placental genomic DNA library. The in...

Journal: :Nucleic acids research 1988
H P Zassenhaus T J Hofmann R Uthayashanker R D Vincent M Zona

The nuclear gene from Saccharomyces cerevisiae that encodes the major mitochondrial nuclease was cloned. Gene sequences were identified from a lambda gt11 library by antibodies specific to the mitochondrial nuclease. DNA from the phage recombinant was used to isolate the entire nuclease gene from a plasmid library. Yeast strains containing the nuclease gene on a multicopy plasmid vector overpro...

Journal: :Applied and environmental microbiology 2005
Binh Nguyen Robert M Bowers Thomas M Wahlund Betsy A Read

The marine coccolithophorid Emiliania huxleyi is a cosmopolitan alga intensely studied in relation to global carbon cycling, biogeochemistry, marine ecology, and biomineralization processes. The biomineralization capabilities of coccolithophorids have attracted the attention of scientists interested in exploiting this ability for the development of materials science and biomedical and biotechno...

Journal: :Biomedical Digital Libraries 2006
Jeffrey Demaine Joel Martin Lynn Wei Berry de Bruijn

BACKGROUND This paper examines how the adoption of a subject-specific library service has changed the way in which its users interact with a digital library. The LitMiner text-analysis application was developed to enable biologists to explore gene relationships in the published literature. The application features a suite of interfaces that enable users to search PubMed as well as local databas...

2018
Reinoud de Groot Joel Lüthi Helen Lindsay René Holtackers Lucas Pelkmans

High-content imaging using automated microscopy and computer vision allows multivariate profiling of single-cell phenotypes. Here, we present methods for the application of the CISPR-Cas9 system in large-scale, image-based, gene perturbation experiments. We show that CRISPR-Cas9-mediated gene perturbation can be achieved in human tissue culture cells in a timeframe that is compatible with image...

Journal: :Bioscience, biotechnology, and biochemistry 2013
Mayank Saraswat Ralph S Grand Wayne M Patrick

It is often desirable to obtain gene libraries with the greatest possible number of variants. We tested two different methods for desalting the products of library ligation reactions (silica-based microcolumns and drop dialysis), and examined their effects on final library size. For both intramolecular and intermolecular ligation, desalting by drop dialysis yielded approximately 3-5 times more ...

Journal: :Nucleic acids research 1990
Z Y Wang Z L Wu Y Y Xing F G Zheng X L Guo W G Zhang M M Hong

Rice waxy gene codes for the starch granule-bound starch synthase (EC 2.4.1.11) (1). To characterize its molecular structure, a rice {Oryza sativa subsp. japonica Hengfeng cultivar) genomic library was constructed in XEMBL-3 vector. Two overlapping genomic clones containing waxy gene sequence were screened and identified from this library by using maize waxy gene DNA as probe (kindly provided b...

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