A novel design is described for an amperometric biosensor based on NAD(P)-dependent glucose dehydrogenase (GDH) combined with a plasma-polymerized thin film (PPF). The GDH is sandwiched between several nanometer thick acetonitrile PPFs on a sputtered gold electrode (PPF/GDH/PPF/Au). The lower PPF layer plays the role as an interface between enzyme and electrode because it is extremely thin, adh...

1. An increase ofglutamic dehydrogenase (GDH) activity in yolk of the hen's egg has been shown to occur in the mid-incubation period. 2. Generally, the levels of GDH in embryonic tissues are less than those of tissues ofthe hen except for the blastoderm and 2-day-old embryo, which have GDH levels as high as those ofsomehen tissues. 3. GDHhasbeenfoundtoincreaseparipa88uwith total protein in the ...

Sequence and structure comparisons of various glutamate dehydrogenases (GDH) and other nicotinamide nucleotide-dependent dehydrogenases have potentially implicated certain residues in coenzyme binding and discrimination. We have mutated key residues in Clostridium symbiosum NAD(+)-specific GDH to investigate their contribution to specificity and to enhance acceptance of NADPH. Comparisons with ...

The NADP-specific glutamate dehydrogenase (NADP-GDH) activity of Aspergillus nidulans was rapidly lost from cultures starved for a carbon source. This loss of NADP-GDH was blocked by protein synthesis inhibitors. Glutamate repressed NADP-GDH but did not cause rapid loss of activity. Since NADP-GDH is involved in the participation of ammonium in the regulation of nitrogen metabolism, the loss of...

The formulation of the Gerasimov-Drell-Hearn sum rule is revisited, showing its connection with other sum rules occurring in electron scattering and discussing the problem of its saturation, both from the theoretical and experimental point of view. The generalisation to other nuclear targets is also reported.

OBJECTIVE To assess a new immunochromatography (ICT) test that detects glutamate dehydrogenase (GDH) antigen and Clostridium difficile toxin A/B simultaneously, and to propose an algorithm for the diagnosis of C. difficile infection (CDI) based on this test. METHODS We analysed 970 stool samples. Discrepant results between GDH and toxin A/B were resolved using toxigenic culture as the referen...

Sensitive, precise, and rapid methods for the measurement of alcohol dehydrogenase (ADH) and glutamate dehydrogenase (GDH) were developed on the Cobas Bio centrifugal analyser. The optimal pH for ADH in caucasians was 9.8. Non-linearity of ADH enzyme activity was observed when samples were diluted in saline; linearity was restored when inactivated serum was used as diluent. ADH was shown to be ...

I . Diets containing varying lcvels of crude protein (CP) in the range 9.5-18.5 "/b with and without supplements of diammonium citrate (DAC) as a source of non-protein nitrogen (KPN) were given to 160 growing chicks from z to 4 weeks of age, and their livers were assayed for glutamate dehydrogenase (GDH) activity. 2. Growth rate and total liver protein were increased by raising the protein leve...

Glucose, the main energy substrate used in the CNS, is continuously supplied by the periphery. Glutamate, the major excitatory neurotransmitter, is foreseen as a complementary energy contributor in the brain. In particular, astrocytes actively take up glutamate and may use it through oxidative glutamate dehydrogenase (GDH) activity. Here, we investigated the significance of glutamate as energy ...

NADP-dependent glutamate dehydrogenase (NADP-Gdh) is the first step in ammonia assimilation pathway in Saccharomyces cerevisiae and the knowledge of its regulation is the key for many biotechnological purposes such as single cell protein production. The regulation of NADP-Gdh activity in Kluyveromyces marxianus cells was evaluated under different ammonia supply in batch cultivations. The result...