Авторами работы проведено генотипирование коров голштинской породы по локусу гена GDF9 в количестве 120 голов методом полимеразной цепной реакции. У исследуемых животных выявлен генетический полиморфизм и были идентифицированы три генетических варианта: AA, AT TT, распространенность которых составила 20,0%, 10,0% 70,0%, соответственно. Отмечается избыточная встречаемость гомозиготного генотипа ...

In this study, we investigated the relationship between the genetic polymorphism of growth differentiation factor 9 (GDF9) genes and the litter size in 384 individuals of five breeds of black goats. Four pairs of primers were designed to detect single nucleotide polymorphism of GDF9 gene in goats by PCR-SSCP. The least square was used to analyze the relation between different genotypes and the ...

Background: In this study we evaluate the effects of different doses of GDF9 with fixed dose of BMP15 on quality of in vitro maturated sheep oocytes. Materials and Methods: Good ovine Cumulus-oocyte complexes (COCs) were first transferred to Pre-IVM medium, and then to three IVM mediums supplemented with 10%v/v BMP15 and: 1) 175 ng/ml 2) 131 ng/ml or 3) 219 ng/ml GDF9. After maturation period, ...

This paper concerns the likely origin of three mutations with large effects on ovulation rate identified in the Belclare and Cambridge sheep breeds; two in the BMP15 gene (FecX(G) and FecX(B)) and the third (FecG(H)) in GDF9. All three mutations segregate in Belclare sheep while one, FecX(B), has not been found in the Cambridge. Both Belclare and Cambridge breeds are relatively recently develop...

BACKGROUND A large number of studies have contributed to understanding the general mechanisms driving ovarian folliculogenesis in humans and show a complex endocrine dialog between the central nervous system, the pituitary and the ovary, integrated by various intraovarian paracrine messages. The role of intraovarian paracrine regulation has acquired more relevance in the recent years owing to t...

for decreasing the numberofbreedingewes onpastures and prevention of demolition pastures, animal breedingprograms are necessary on genes with major effects on litter size in iranian sheep breeds for identify effective candidate genes on these economical traits .the gdf9 gene is one of the most important effective factors on litter size in sheep. the aim of the present study was to identify g2, ...