This paper presents the motivation and design decisions for the Web Services Grid Application Framework (WS-GAF), which is a mapping of Grid architecture requirements onto the Web Services Architecture. The goal for WS-GAF is to describe a framework for building Grid applications that adheres to the principles of service-oriented architectures and utilises existing Web Services technologies. Th...

Near-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes (BphPs) are of great interest for in vivo imaging. They utilize biliverdin (BV) as a chromophore, which is a heme degradation product, and therefore they are straightforward to use in mammalian tissues. Here, we report on fluorescence properties of NIR FPs with key alterations in their BV binding sites. BphP1-FP...

Thousands of genes in Drosophila have Pol II paused in the promoter proximal region. Almost half of these genes are associated with either GAGA factor (GAF) or a newly discovered factor we call M1BP. Although both factors dictate the association of Pol II at their target promoters, they are nearly mutually exclusive on the genome and mediate different mechanisms of regulation. High-resolution m...

Tissue fixation in phosphate buffered formalin (PBF) remains the standard procedure in histopathology, since it results in an optimal structural, antigenic and molecular preservation that justifies the pivotal role presently played by diagnoses on PBF-fixed tissues in precision medicine. However, toxicity of formaldehyde causes an environmental concern and may demand substitution of this reagen...

The expression of genes required for the synthesis of molybdenum nitrogenase in Azotobacter vinelandii is controlled by the NifL-NifA transcriptional regulatory complex in response to nitrogen, carbon, and redox status. Activation of nif gene expression by the transcriptional activator NifA is inhibited by direct protein-protein interaction with NifL under conditions unfavorable for nitrogen fi...

OBJECTIVE The study evaluated the effectiveness of day treatment for poorly functioning patients with personality disorders who participated in day treatment consisting of analytically oriented and cognitive-behavioral therapy groups as part of a comprehensive group therapy program. METHODS At admission, discharge, and one year after discharge, patients completed the Global Severity Index (GS...

This paper introduces GAF, a grounded annotation framework to represent events in a formal context that can represent information from both textual and extra-textual sources. GAF makes a clear distinction between mentions of events in text and their formal representation as instances in a semantic layer. Instances are represented by RDF compliant URIs that are shared across different research d...

CONCLUSIONS • Despite extensive use of the GAF to evaluate functioning in patients with schizophrenia, this is the first empirical documentation of minimal clinically important difference thresholds, using distribution-based and anchor-based approaches. • Results range from 2.9 (within-patients score approach) to 11.7 (SD approach). • We suggest using a MCID of 10, based on the SEM approach. Th...

Background Comorbid mental disorders in patients with coronary artery disease (CAD) are common and associated with adverse somatic outcomes. However, data on utilization rates of mental health care and treatment efficiency are scarce and inconsistent, which we tried to remedy with the present preliminary study on Northern German CAD patients. Method A total of 514 German CAD patients, as diag...

In order to apply the advantages of image recognition for fault diagnosis using convolutional neural network (CNN), it is necessary convert one-dimensional (1D) signal data into two-dimensional (2D) images. Traditional signal-based conversion methods face challenges complex feature extraction process, high dependence on expert knowledge and poor repeatability, which hinder timely accurate diagn...