With regard to human cloning or artificial human reproduction – and contrary to the opinions of Sunni scholars - Shiite thinkers have not held a unified position. After having surveyed a number of Shiite fatwas and analyses on the subject, this essay will classify them into four groups. The first group states that we are granted absolute permission to engage in human cloning; while the second g...

In the present study, we examined the feasibility of using recombinant antibodies containing murine variable regions and human constant regions as calibrators or controls in immunoassays. As a model system, we chose the Abbott IMx Toxo immunoglobulin M (IgM) and Toxo IgG assays designed to detect antibodies to Toxoplasma gondii. Two mouse monoclonal antibodies were selected based on their react...

24 Buchanan, S. K., Smith, B. S., Venkatramani. L., Xia, D., Esser, L.. Palnitkar, M.. Chakraborty, R, van der Helm, D. and Deisenhofer, J. ( I 999) Nat. Struct. Biol. 6, 5 6 6 3 Jalal, M. A. F. and van der Helm, D. (1989) FEBS Lett. 243, 366370 Sambrook 1.. Fritsch, E. F. and Maniatis, T. ( I 989) in Molecular Cloning: a Laboratory Manual, 2nd edn., Cold Spring Harbor Press, Cold Spring Harbor...

Adipose triglyceride lipase (ATGL), a newly identified lipase, is a rate-limiting enzyme for triglyceride hydrolysis in adipocytes. The regulatory proteins involved in ATGL-mediated lipolysis in fat tissue are not fully identified and understood. The G(0)/G(1) switch gene 2 (G0S2) is an inhibitor of ATGL activity by interacting with ATGL through the hydrophobic domain of G0S2. Here, for the fir...

In the random graph G(n, p) with pn bounded, the degrees of the vertices are almost i.i.d. Poisson random variables with mean λ := p(n− 1). Motivated by this fact, we introduce the Poisson cloning model GPC(n, p) for random graphs in which the degrees are i.i.d. Poisson random variables with mean λ. We first establish a theorem that shows that the new model is equivalent to the classical model ...

We report the cloning of a low-molecular-weight GTP-binding protein that appears to be the first member of a new class of G proteins. This G protein was cloned from the HT4 neural cell line and has the closest homology to the rab, sec4, and ypt1 members of the low-molecular-weight (LMW) G-protein family. The amino acid sequence identity is only 30% with these other LMW G proteins, but in the fo...

After the appearance of no-cloning theorem, approximate quantum cloning machines (QCMs) has become a well-studied subject in information theory. Among several measures to quantify performance QCM, single-qudit fidelity and global have been most widely used. In this paper we compute optimal for phase-covariant via semidefinite programming optimization, thereby completing remaining gap previous r...

Abstract Generation of new DNA constructs is an essential process in modern life science and biotechnology. Modular cloning systems based on Golden Gate cloning, using Type IIS restriction endonucleases, allow assembly complex multipart from reusable basic parts a rapid, reliable automation-friendly way. Many such toolkits are available, with varying degrees compatibility, most which aimed at s...

objective: leishmaniasis is caused by parasitic protozoa of the genus leishmania which in the infected host is obligate intracellular parasite. lack gene is conserved among related leishmania species. lack is the immuno-dominant antigen of l.major which is considered as the most promising molecule for a recombinant or dna vaccine against leishmaniasis. materials and methods: in this study the ...

2. Pournaras S, Tsakris A, Maniati M, Tzouvelekis LS, Maniatis AN. Novel variant (bla(VIM-4)) of the metallo-β-lactamase gene bla(VIM-1) in a clinical strain of Pseudomonas aeruginosa. Antimicrob Agents Chemother 2002;46:4026–8. 3. Toleman MA, Rolston K, Jones RN, Walsh TR. Molecular characterization of VIM-4, a novel metallo-β-lactamase isolated from Texas: report from the CANCER surveillance ...