This study was performed to determine whether alloxan treatment of rats alters levels of the terminal carbohydrate residues L-fucose and sialic acid of serum glycoproteins. Results indicate that in the uncompensated diabetic animal a chronic depression of serum sialic acid level occurs with no apparent alteration in the level of L-fucose. The depression in sialic acid level may be attributed in...

Recent studies from several laboratories have provided evidence that cell surface complex carbohydrates play key roles in the regulation of developmentally relevant signal transduction events. The demonstration that Fringe, a known modifier of Notch function, is a fucose-specific N-acetylglucosaminyltransferase provided strong evidence that the Notch signaling pathway could be regulated by alte...

Some physicochemical properties of the L-fucose-binding lectin from the serum of the European eel (Anguilla anguilla) were determined. The lectin is a dimer composed of identical subunits of Mr approx. 40000. In agreement with previous results [Horejsí & Kocourek (1978) Biochim. Biophys. Acta 538, 299-315], the subunit was shown to comprise two non-glycosylated polypeptides of Mr approx. 20000 ...

Guanosine 5’-diphospho-β-l-fucose (GDP-Fuc) is an important and expensive nucleotide sugar only available in low quantities. We established two processes for the gram-scale production of GDP-Fuc with multi-enzyme cascades. used bifunctional enzyme fucokinase/GDP-fucose pyrophosphorylase from Bacteroides fragilis either ATP-regeneration system using polyphosphate or excess ATP. The optimized mod...

Fucose is an L-configuration sugar found abundantly in the mammalian gut. It has long been known to be induced there by the presence of bacteria, but only recently have some of the molecular mechanisms behind this process been uncovered. New work suggests that fucose can have a protective role in both gut-centered and systemic infection and inflammation. This review highlights recent studies sh...

glycoconjugates and their programmed changes during the course of development in the cell-surface as well as in the extracellular matrix, are known to affect cell differentiation, cellular interaction and other developmental phenomena during embryogenesis. the purpose of this study was to localize n-acetylgalactosamin as well as fucose-containing glycoconjugates in situ during thymus developmen...

L-Fucose is a monosaccharide found as a component of glycoproteins and cell wall polysaccharides in higher plants. The MUR1 gene of Arabidopsis thaliana encodes a GDP-D-mannose 4,6-dehydratase catalyzing the first step in the de novo synthesis of GDP-L-fucose from GDP-D-mannose (Bonin et al. 1997, Proc. Natl Acad. Sci. USA, 94, 2085-2090). Plant genes encoding the subsequent steps in L-fucose s...

Invasion specificity of Eimeria stiedai sporozoites in cultured cells was examined. Intracellular sporozoites were observed in hepatobiliary epithelial cells, as early as 3 hours post inoculation (p.i.) but the infection rate was monitored for 6 hours. No intracellular parasites were found in rabbit parenchymal hepatocytes and rabbit kidney cells, even on prolonged culturing. In the hepatobilia...