The experiments were carried out to evaluate a simple method of freezing rabbit spermatozoa. The effect of glucose, lactose or sucrose on post-thawing sperm quality was studied. The results showed that addition of sucrose improved motility and acrosomal integrity (57% of progressive motile spermatozoa vs 43% and 42%, and 68% of normal acrosome vs 56 and 57%, sucrose, lactose and glucose, respec...

Ferti l i ty of semen frozen in a mechanica~ forced-vapor freezer was compared with that frozen in static nitrogen (N) vapor. Semen from 10 Holstein bulls was extended in milk-10% glycerol at 30 x 106 progressively motile spermatozoa/ml and packaged in .5-ml French straws. Straws were divided into three equal g roups ' bu l l l ' co l l ec t ion day -I and frozen in a mechanical freezer at full...

This study was carried out to investigate effects of copper sulphate (CuSO4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and DNA damage, after semen dilution and cryopreservation. Semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. A total number of 25 samples were u...

Environmental influences on reproduction and semen production in the buck, the problem of interaction between seminal plasma and egg yolk or milk constituents in diluent, liquid storage and processing of semen for freezing are discussed. A review is given on the use of frozen-thawed semen for artificial insemination (AI) in spontaneous and induced oestrus and factors influencing the fertility.

This paper describes a procedure of laparoscopic insemination (LAI) performed in 42 Ile de France ewes with deep-frozen rams’ semen. Frozen semen doses was imported from France, originating from two rams (A and B), and used intermittently in three LAI cycles on different females. The ewes were examined ultrasonographically for pregnancy, two months following insemination and data of lambing wer...

Artificial insemination (AI) and semen freezing have become services available to dog owners worldwide, and the demand for services to freeze semen is increasing. In other canids such as the fox, the fur industry utilizes fresh or frozen semen to artificially inseminate vixens to produce pelts. Clearly, AI facilitates the use of a male to sire several females by diluting the ejaculate, increase...

A continuous decline in the number and range of capercaillie (Tetrao urogallus L.) in many European countries can be observed, mostly due to habitat destruction by human activity, unecological forestry management, and increased density of natural predators. Ex situ in vitro gene banks provide a unique opportunity to preserve the genetic material for future generations. Simple and effective cryo...

Background Although several studies confirmed positive effects of fish oil on semen quality, the antioxidant status in omega-3 supplemented diets of several studies is a fuzzy point. The aim of this study was to investigate the effect of dietary fish oil and (or) vitamin E supplementation on cryopreserved sperm in Mehraban rams. MaterialsAndMethods Sixteen fertile rams were randomly allotted to...

Experiments were designed to determine when, during the cryopreservation process, sperm lose fertilizing capacity and whether the cryoprotectant, methyl cellulose (MC), could be used in combination with glycerol to cryopreserve sperm and remain in the inseminate without reducing fertility. Semen diluted in Minnesota Avian extender (MNA) and inseminated immediately had greater fertility (75%) th...