This study was designed to investigate the effect of vitrification and post-thaw survival and chromosomal aberrations caused by vitrification of vitrified 8-cell mouse embryos in comparison with a control group. To this purpose the survival rate and the frequency of chromosomal aberrations were assessed in frozen-thawed 8-cell mouse embryos after various storage durations in the presence of eth...

background: we performed this study to evaluate use of fresh and frozen sperm samples in non-obstructive azoospermia microdissection testicular sperm extraction (micro-tese-icsi) treatment. methods: we performed a total of 82 consecutive in vitro fertilization (ivf) cycles at fertijin ivf center in istanbul, turkey from january 2010 to march 2012. in 43 participants we used fresh sperm and froz...

BACKGROUND This study aimed to investigate the effects of vitrification and slow freezing on actin, tubulin, and nuclei of in vivo preimplantation murine embryos at various developmental stages using a Confocal Laser Scanning Microscope (CLSM). MATERIAL/METHODS Fifty female mice, aged 4-6 weeks, were used in this study. Animals were superovulated, cohabitated overnight, and sacrificed. Fallop...

Background: Couples receiving IVF treatment may choose to have embryos frozen, with the aim of creating a pregnancy and ultimately a live birth in future. Problems can arise when couples separate. Under UK law, embryos can only be transferred with the consent of both genetic parents. The issue came to public prominence in the case of Natallie Evans, who lost the opportunity to have her own gene...

Controlled ovarian hyperstimulation (COH) affects adversely implantation following in vitro fertilization and embryo transfer. COH with gonadotropins alters endometrial receptivity and luteal function. In addition periovulatory endometrial characteristics in stimulated cycles are considerably different compared with the natural cycles, and periovulatory secretory transformation is consistently ...

OBJECTIVE To evaluate the outcome of frozen-thawed ET using embryos previously biopsied for preimplantation genetic diagnosis during a fresh ET cycle. DESIGN Prospective evaluation. SETTING Assisted reproductive biology program. PATIENT(S) A 31-year-old, G4, P1, TAB1, SAB2 carrier of a balanced RT 45,XX der(14;21)(q10;q10) translocation. INTERVENTION(S) Preimplantation genetic diagnosis...

nowadays gamete and embryo freezing is an appropriate approach for preserving of genetic traits in laboratory animals, rare and endangered species. frozen cells are suitable replace for actively breeding animals colony. the aim of this study was to perserve laboratory mouse embryo, using vitrification method and comparing effect of two cryoprotectants, glycerol-sucrose(gs) and ethylene glycol-f...