this study was undertaken to compare the efficiency of fresh and frozen-thawed buck semen on in vitro fertilization (ivf) of goat oocytes. cumulus oocytes complexes (cocs) were collected by aspiration of 2-6 mm diameter follicles, which were obtained from slaughterhouse. upon grading, only normal quality cocs were maturated in tcm-199 for 48 hours. the percentage of cocs reached to the m-ii sta...

This chapter describes some simple standard operating procedures for the regular collection of samples from surgical resections and their rapid preservation by freezing for long-term cryogenic storage.

semen from six adult male black bengal goats (capra hircus) was collected to compare the fresh and frozen-thawed semen quality and investigate its relationship with the fertility. each collected samples was divided into two parts: one part of semen was used as fresh and another part was filled into 0.50 ml straws, sealed, cooled (5 ˚c) and equilibrated for freezing.in both cases, semen was dilu...

Fractal analysis of the morphology of ice crystal particles in frozen food was attempted for frozen soybean curd (tofu). From a microscopic image analysis of the ice crystal particles, it was found that the perimeter of the ice crystal particles could be recognized as a fractal. Effects of the storage time and storage temperature on the fractal dimension (d(p)) of the perimeter of the ice cryst...

Semen from six adult male Black Bengal goats (Capra hircus) was collected to compare the fresh and frozen-thawed semen quality and investigate its relationship with the fertility. Each collected samples was divided into two parts: One part of semen was used as fresh and another part was filled into 0.50 ml straws, sealed, cooled (5 ˚C) and equilibrated for freezing.In both cases, semen was dilu...

gums are the most important stabilizers that are used in ice cream and other frozen dairy dessert industries. in this study, effect of different levels of guar gum (0, 0.1, 0.2 and 0.3%) and arabic gum (0, 0.1, 0.3 and 0.5%) on rheological behavior of frozen yogurt was investigated. the rheological behavior of samples showed that all samples exhibit shear thinning behavior and power law model f...

purpose: to compare the clinical and paraclinical outcomes following deep anterior lamellar keratoplasty (dalk) using frozen versus fresh corneas in patients with keratoconus. methods: in this prospective, comparative interventional study, 16 frozen and 19 fresh corneas were used during dalk in keratoconus patients. big bubble technique was used in all patients. the two groups were compared at ...

Ultrathin frozen sections of biological tissues for electron microscopy provide certain advantages in cytochemical studies in which the penetration of cells by large molecules is necessary and in morphological studies of cellular constituents which are dissolved by the reagents employed in routine plastic embedding . The recent introduction of several types of commercially available cryo-ultram...

PURPOSE To describe a novel methodology by which to measure the Hirschberg ratio (HR) in infants. The methodology does not require fixation on specific points, and measurements are made while infants look naturally at a display. METHODS The HR is calculated automatically from measurements of the direction of the optical axis, the position of the pupil center, and corneal reflexes in video ima...