The sonication method produced a quantitatively higher release of buffalo sperm hyaluronidase than the freeze-thaw technique. The released enzyme constituted 10 p. 100 of the total enzymatic activity of the fresh semen. Seminal plasma hyaluronidase activity was not correlated with the motility score of the semen sample. Unlike cattle semen, the seminal plasma enzyme level in buffalo semen store...

The effect of different phases of a freezing protocol on the fertilising ability of rabbit spermatozoa was evaluated. An extender containing 1.75 M DMSO and 0.05 M sucrose (final concentration) was used to freeze rabbit sperm. In the first experiment, the results obtained with fresh and cooled (5 degrees C for 45 min) sperm were compared; no differences were observed between fresh and cooled se...

Cryopreservation has the capacity to extend spermatozoa's lifespan and viability. In addition, the semen samples can be collected, preserved and stored or sent to distant locations and still be used long after the death of the semen donor. In this study for the vitrification of dog sperm (fresh and swum-up sperm), different cryopreservation mediums on the basis of glycerol, milk and egg yolk we...

The objective of this experiment was to evaluate spermatological parameters and fertilizing ability of short term stored semen in different extenders from endemic Abant trout (Salmo trutta abanticus T,1954). Semen was collected from 30 adult males by the hand stripping method without anesthesia. Having determined the main spermatological properties (volume, motility, movement duration, concentr...

BACKGROUND Long-chain polyunsaturated fatty acids (PUFAs) of the omega-3 family are important for sperm membrane integrity, sperm motility and viability. There are evidences to suggest that dietary supplementation with omega-3 fatty acids affects reproduction in men and males of different animal species. Therefore, the aim of current study was to investigate changes in the quality parameters of...

The freezability of semen collected during the low (May-July) and the peak (September-November) breeding seasons from 18 Nili-Ravi buffalo bulls of young (3-4 years), adult (6-8 years) and old ( 1215 years) age groups was studied. After extension in lactose.:..fructose-egg-yolk-glycerol extender, semen samples were frozen and stored in liquid nitrogen. Post-thaw motili�y and liveability of sper...

Somatic cells were isolated from cryopreserved semen of 4 buffalo bulls, 3 of which had died over 10 years earlier, and were established in culture. The cells expressed cytokeratin-18, keratin and vimentin indicating that they were of epithelial origin. The cells were used as nuclear donors for hand-made cloning for producing buffalo embryos. The blastocyst rate and quality, as indicated by apo...

Introduction While conception rates following cervical insemination of synchronised ewes with fresh semen are generally around 60-70%, conception rates with frozen-thawed semen are unacceptably low (<20% generally). These poor conception rates following cervical insemination with frozen-thawed semen appear to stem from two inter-related factors which are additive in their negative impacts: (a) ...