BACKGROUND During the previous century Norway had a high incidence of tuberculosis, but no molecular epidemiological studies could be performed and these previously epidemic strains have been disappearing during the last decades. Currently, tuberculosis among native Norwegians is in the elimination phase, and it is still not known what type of M. tuberculosis was so efficiently controlled durin...

Source and Description of Clone: phPAH247, a 2.4 kb EcoSl fragment subcloned into pBR322 from a human liver cDNA library (1).

The discrimination of Leishmania species from patient samples has epidemiological and clinical relevance. In this study, different gene target PCR-restriction fragment length polymorphism (RFLP) protocols were evaluated for their robustness as Leishmania species discriminators in 61 patients with cutaneous leishmaniasis. We modified the hsp70-PCR-RFLP protocol and found it to be the most reliab...

Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and ...

In a number of countries, tuberculosis (due to infection with Mycobacterium bovis) is a significant health problem of captive deer. This paper describes outbreaks of bovine tuberculosis in sika deer (Cervus nippon) on two farms in Ireland and the methods used to control the disease. On Farm A, infection was first detected during 1993. The infection was eradicated using a programme of test and r...

A rapid two-step identification scheme based on PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the 16S rRNA gene was developed in order to differentiate isolates belonging to the Campylobacter, Arcobacter, and Helicobacter genera. For 158 isolates (26 reference cultures and 132 clinical isolates), specific RFLP patterns were obtained and species were successfully identified...

SOURCE/DESCRIPTION: A I.i KB BlBa 111, EcoRi fragment from a pUC clone containing a 3.8 kb r Hind III fragment of the human MOX-1 gene'. The antigen coded for by the MOX-1 gene is thought to belong to the immunoglobulin super family: r the 1.1 kb fragment contains the exon for the C-like domain.

Two RFLPs near HOX2@/NGFR at locus D17S444E J.L.Kennedy, W.G.Honer, C.A.Kaufmann, J.A.Martignetti, J.Brosius and K.K.Kidd Clarke Institute of Psychiatry, 250 College St, Toronto, Ontario, M5T 1R8, Canada, 1 Department of Psychiatry, Columbia University, 722 West 168 St, New York, NY 10032, Mount Sinai School of Medicine, One Gustave Levy PL, New York, NY 10029 and Department of Genetics, Yale U...

Towards assessing the genetic diversity and occurrence of Trichoderma species from the Indian region of Indo-Burma Biodiversity hotspot, a total of 193 Trichoderma strains were isolated from cultivated soils of nine different districts of Manipur comprising 4 different agroclimatic zones. The isolates were grouped based on the morphological characteristics. ITS-RFLP of the rDNA region using thr...

In Errachidia province, the incidence of cutaneous leishmaniasis (CL) has increased over the past decade and it was higher in 2010 (860.34 per 100,000 inhabitants), with 3445 cases. The number of cases declined sharply and decreased from 3445 cases in 2010 to 8 cases in 2014 following the control action plan interventions. The total of patients was diagnosed only on clinical basis and the lesio...