A low-molecular-mass modulator protein having a molecular mass of about 12 kDa has been purified from rat brain cytosol following gel filtration and FPLC/Mono Q anion-exchange chromatographic separation. A number of protein fractions were obtained from an FPLC column when eluted with a 0.1 M NaCl hold gradient. One fraction (peak no. 5) was found to stimulate Ca2+,Mg2+-ATPase but inhibit Ca2+-A...

OBJECTIVE The aim of the study was to investigate LDL modifications by cultured human umbilical vein endothelial cells (HUVEC) from women smokers and non-smokers. METHODS Modifications of LDL by HUVEC were studied by determining the values of thiobarbituric acid-reactive substances (TBARS) and the percentage of the most electronegative oxidized LDL fraction (fraction C) by using an ion-exchan...

OBJECTIVE Elevated triglyceride (TG) is the major plasma lipid abnormality in obese and diabetic patients and contributes to cardiovascular morbidity in these disorders. We sought to identify novel mechanisms leading to hypertriglyceridemia. Resistance to negative feedback signals from adipose tissue in key central nervous system (CNS) energy homeostatic circuits contributes to the development ...

amphibians have a large variety of antimicrobial peptides that serve as natural innate barriers limiting microbial infection or, in some instances, act as an integral component in response to inflammation or microbial infection. a novel peptide with antibacterial effects and without hemolytic activity was purified from skin secretions of rana ridibunda by multisteps cation-exchange fplc, revers...

BACKGROUND Peptides derived from brain natriuretic peptide (BNP) precursor (proBNP), BNP, and the N-terminal fragment of proBNP (NT-proBNP) are used as biomarkers of heart failure. It remains unclear which forms of these peptides circulate in blood and which forms are measured by assays for these natriuretic peptides. METHODS To design assays for immunodetection of proBNP, NT-proBNP, and BNP,...

Aim. To search fibrinogenolytic enzymes among protein components of Vipera renardi snake venom. Methods. Venom V. as the lyophilized powder was supplied by Trypillia serpentarium. It dissolved in 0.05 M Tris-HCl buffer pH 8.3 and fractionated on Superdex G-75 using FPLC system Acta Prime. Peaks were tested for their ability to directly cleave fibrinogen. Hydrolytic products analyzed SDS-PAGE. E...

Proteases possuem um papel fundamental na economia, estando presentes em diversos setores industriais. Dentre os fungos conhecidos por produzir proteases, destaca-se o gênero Aspergillus. Desta forma, objetivo deste trabalho foi proteases a partir de fermentação estado sólido Aspergillus ochraceus, purificar, caracterizar e avaliar seu potencial produção enzima colagenolítica fibrinolítica. Par...

Two novel extracellular keratinases were produced by Actinomadura keratinilytica strain Cpt20. Both enzymes purified to homogeneity using heat-treatment (60 °C for 30 min) and ammonium sulfate salt fractionation (40 %–70 %), followed anion-exchange chromatography with fast protein liquid (FPLC) system. The keratinases, designated as KERA-71 KERB-19, are monomeric named according their molecular...

Amphibians have a large variety of antimicrobial peptides that serve as natural innate barriers limiting microbial infection or, in some instances, act as an integral component in response to inflammation or microbial infection. A novel peptide with antibacterial effects and without hemolytic activity was purified from skin secretions of Rana ridibunda by multisteps cation-exchange FPLC, revers...