BACKGROUND Thermophilic campylobacters, particularly Campylobacter jejuni and C. coli are the main agents of human campylobacteriosis. Campylobacter contaminated chicken products is the most important source of foodborne gastroenteritis. Evaluation of genetic diversity among Campylobacter population is critical for understanding the epidemiology of this bacterium and developing effective contro...

Mycobacterium tuberculosis sputum isolates from 38 patients, obtained in the first 6 months of 1997 in Havana, Cuba, were characterized by IS6110 restriction fragment length polymorphism (RFLP) analysis and the double-repetitive-element PCR (DRE-PCR) method. Among 41 strains from 38 patients, 24 and 25 unique patterns, and 5 and 4 cluster patterns, were found by the RFLP and DRE-PCR methods, re...

Aim: Chital and sambar are the common wild small herbivores, which are vulnerable to poaching for their meat. Many times poachers claim the wild meat to be that of goat or sheep. Hence, authentic evidences are required to stop such wildlife crime. The present investigation was carried out to study the species specific PCR-RFLP patterns for meat identification of chital and sambar and then diffe...

Amplified-fragment length polymorphism (AFLP) analysis with the endonucleases BglII and MfeI was used to genotype 91 Campylobacter jejuni subsp. jejuni strains from outbreaks and sporadic cases. AFLP-generated fragments were labeled with fluorescent dye and separated by capillary electrophoresis. The software packages GeneScan and GelCompar II were used to calculate AFLP pattern similarities an...

We here identified for the first time the presence of Mycobacterium avium paratuberculosis (MAP) sheep (S) strain in Argentina. IS900 polymerase chain reaction (PCR) was positive. The S strain was compared with MAP cattle (C) strains by using IS1311 PCR-restriction endonuclease analysis (PCR-REA), multiplex PCR and restriction fragment length polymorphism (RFLP) analysis.

Diabetes mellitus and obesity are very similar in terms of pathogenesis pathophysiology. Most obese patients have adipose tissue dysfunction caused by genetic environmental factors. Adipose is the source adipokines secreted from adipocytes. Vaspin, Visfatin Chemerin discovered recent years. The study consists 43 non-diabetic 51 diabetic individuals. PCR-RFLP method agarose gel electrophoresis t...

Neovascular Age-related Macular Degeneration (nAMD) is one of the major factors for blindness and impaired visual acuity in elderly people. The aim this study was CFH gene screening Age-Related patients Indonesia. This performed 106 AMD 104 controls genomic markers Complement Factor H ( ). diagnosis carried out by retinal specialists based on color fundus photography optical coherence tomograph...

Introduction: The TP53 and CHEK2 genes have been described as breast cancer susceptibility some of their polymorphisms associated with an increased risk in certain populations.Aim: objective this study was to investigate the p.R72P PIN3 Ins16bp (TP53) I157T (CHEK2) mutation developping cancer. Methods: This case-control had enrolled 144 participants including 65 cases (breast patients) 79 contr...

This study was carried out on 114 Brown Swiss cattle reared in intensive conditions at Ataturk University Food and Livestock Application Research Center the private farm found Erzurum province. Genotypic structures were examined terms of Insulin-like growth factor (IGF)-II gene locus distribution genotypes allele frequencies concerning genes determined. The identified associated with milk yield...

b-lactoglobulin (coded by the b-lg gene) is the major milk whey protein in ruminants. studies have shown that this protein is polymorphic in many breeds of sheep as a result of a single base pair substitution in the b-lg gene that also gives rise to rsai restriction fragment length polymorphism (rflp). blood samples were collected from 391 animals belonging to 5 iranian and 6 russian sheep bree...