The fluorescent compound trisodium, 3-hydroxy-5,8,10-pyrenetrisulfonate (PTS) was used to quantify the apoplastic flux through red pine (Pinus resinosa Ait.) root systems-that portion of the total water flux reaching the xylem without ever crossing a semipermeable membrane. Flow was induced by pressure through detopped root systems, and by transpiration through intact seedlings. Apoplastic flux...

[1] Understanding the processes involved with the advance of lava flows is critical for improving hazard assessments at many volcanoes. Here, we describe the application of computer vision and oblique photogrammetric techniques to visible and thermal images of active ‘a‘ a flows in order to investigate distal flow processes at Mount Etna, Sicily. Photogrammetric surveys were carried out to prod...

A numerical model of axisymmetric convection in the presence of a vertical magnetic flux bundle and rotation about the axis is presented. The model contains a compressible plasma described by the nonlinear MHD equations, with density and temperature gradients simulating the upper layer of the sun’s convection zone. The solutions exhibit a central magnetic flux tube in a cylindrical numerical do...

W detail here a protocol using tandem-tagged mCherry-EGFPLC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on their relative levels of autophagic flux. This robust and sensitive method measures autophagic flux rather than autophagosome number and is an important addition to the autophagy researcher’s array of too...

As a raindrop falls from the atmosphere, over vegetation and forest canopies, and enters soils and streams it experiences a dynamic exchange of carbon constituents with the surrounding environment. Understanding the magnitude and dynamics of carbon export in above and below ground flow paths is critical for constraining the influence of terrestrial and aquatic ecosystems on global carbon cyclin...

A characterization and optimization of the in vitro transdermal iontophoretic transport of 5-hydroxy-2-(N,N,-di-n-propylamino)tetralin (5-OH-DPAT) is presented. The utility of acetaminophen as a marker of electroosmotic flow was studied as well. The following parameters of iontophoretic transport of 5-OH-DPAT were examined: drug donor concentration, electroosmotic contribution, influence of co-...

We detail here a protocol using tandem-tagged mCherry-EGFP-LC3 (C-G-LC3) to quantify autophagic flux in single cells by ratiometric flow cytometry and to isolate subpopulations of cells based on their relative levels of autophagic flux. This robust and sensitive method measures autophagic flux rather than autophagosome number and is an important addition to the autophagy researcher's array of t...

We report results from analog laboratory experiments, in which a large-scale flow is imposed upon natural convection from a hot boundary layer at the base of a large tank of corn syrup. The experiments show that the subdivision of the convective flow into four regions provides a reasonable conceptual framework for interpreting the effects of large-scale flow on plumes. Region I includes the are...