Fluorescent-labeled molecules have been used extensively for a wide range of applications in biological detection and diagnosis. A new form of highly luminescent and photostable nanoparticles was generated by doping the fluorescent dye tris(2'2-bipyridyl)dichlororuthenium(II)hexahydrate (Rubpy) inside silica material. Because thousands of fluorescent dye molecules are encapsulated in the silica...

• While living species require Cu 2+ , they are particularly sensitive to high levels of which can be toxic and even lethal. mostly reaches organisms via aquatic environments. Thioflavin T has the ability form a G-quadruplex complex with guanine rich DNA sequences where fluorescence intensity is produced as result this. High fluorescent by drops when binds . The fluorescent-changing mechanism w...

Pyrrolo-C (PC), or 3-[beta-D-2-ribofuranosyl]-6-methylpyrrolo[2,3-d]pyrimidin-2(3H)-one, is a fluorescent analog of the nucleoside cytidine that retains its Watson-Crick base-pairing capacity with G. Due to its red-shifted absorbance, it can be selectively excited in the presence of natural nucleosides, making it a potential site-specific probe for RNA structure and dynamics. Similar to 2-amino...

A novel fluorescent method for the detection of single nucleotide polymorphism (SNP) was developed using a hairpin DNA containing nucleotide base analog pyrrolo-deoxycytidine (P-dC) as a fluorescent probe. This fluorescent probe was designed by incorporating a fluorescent P-dC into a stem of the hairpin DNA, whose sequence of the loop moiety complemented the target single strand DNA (ss-DNA). I...

We report the development of a fluorescence-based immunoassay technique relying on the physical phenomena of random number fluctuations and diffusion, which we review. By determining the autocorrelation of the fluctuations in the fluorescent intensity, this methid is able to measure the amount of labeled antigen or antibody that is bound to micrometer-sized carrier particles in solution. The pr...

Stimulated emission depletion (STED) is the key optical technology enabling super-resolution microscopy below the diffraction limit. Here, we demonstrate that modulation of STED in the time domain, combined with properly designed lock-in detection, can radically enhance the contrast of fluorescent images of strongly autofluorescent biotissues. In our experiments, the temporally modulated STED t...

With this research we set out to develop a coumarin-based novel fluorescent probe NTR-AHC for the detection of biological thiols and nitroreductase (NTR). Probe was constructed by attaching NTR trigger moiety (p-nitrobenzyl) biothiol acceptor (maleic acid monoamide), core fluorophore AHC. In presence both glutathione (GSH) NTR, produced >8-fold increase in fluorescence intensity at 463 nm.

Abstract We report a series of ( Z )-2-acetyl-4-benzyliden-1-methyl-1 Н -imidazol-5(4 )-ones with pronounced solvent-dependent intensity fluorescence variation. The introduction the 2-acetyl group allows one to shift absorption and emission maxima long-wavelength region. have shown that these compounds can be used for staining endoplasmic reticulum fluorescent microscopy.