rhabditidae is a family of free-living nematodes. free living nematodes due to their active movement and resistance to chlorination, do not remove in conventional water treatment processes thus can be entered to distribution systems and cause adverse health effects. ultraviolet radiation (uv) can be used as a method of inactivating for these organisms. this cross sectional study was done to inv...

AIMS In this study, a loop-mediated isothermal amplification (LAMP) assay has been developed and evaluated for the rapid and sensitive detection of Verticillium dahliae Kleb., the causal agent of vascular wilts in many economically important crops. METHODS AND RESULTS LAMP primers were designed based on a previously described RAPD marker, and the LAMP assay was applied for direct detection of...

OBJECTIVES Recent research suggests that lysosomal-associated membrane protein-2 (LAMP-2) could be one of the target antigens in the pathogenesis of vasculitides. We established a transgenic rat model, env-pX rats, with various vasculitides including cutaneous vasculitis. Human primary cutaneous vasculitis includes cutaneous polyarteritis nodosa (CPN) and Henoch-Schönlein purpura (HSP). We meas...

Ag processing is a critical step in defining the repertoire of epitope-specific immune responses. In the present study, HIV-1 p55Gag Ag was synthesized as a DNA plasmid with either lysosomal-associated membrane protein-1 (LAMP/gag) or human dendritic cell-LAMP (DC-LAMP/gag) and used to immunize mice. Analysis of the cellular trafficking of these two chimeras demonstrated that both molecules col...

The lack of rapid, affordable, and accurate diagnostic tests represents the primary hurdle affecting malaria surveillance in resource- and expertise-limited areas. Loop-mediated isothermal amplification (LAMP) is a sensitive, rapid, and cheap diagnostic method. Five species-specific LAMP assays were developed based on 18S rRNA gene. Sensitivity and specificity of LAMP results were calculated as...

In this paper a compact representation, 3D Layered, Adaptive-resolution and Multi-perspective Panorama (LAMP), is proposed for representing large scale and 3D scenes with occlusion. Two kinds of 3D LAMP representations are constructed, i.e. the relief-like LAMP and the image-based LAMP, both of which concisely represent almost all the information from a long image sequence. The relief-like LAMP...

Abstract Spectral irradiance scale in the wavelength range from 250 nm to 2500 was realized at National Institute of Metrology (NIM) on basis a large area tungsten carbide–carbon (WC-C) high temperature fixed point blackbody, which is composed 14 mm diameter WC-C cell and variable blackbody BB3500MP as furnace. A series 1000 W FEL halogen lamps were used transfer standards. The new spectral com...

Background: Salmonella can cause serious human gastroenteritis and is frequently isolated from various food samples. The cell culturing, immunoassay, and polymerase chain reactions (PCR) are the current methods to detect such pathogenic agents. However, these methods are time-consuming and labor-intensive, and thus unavailable for rapid-monitoring of Salmonella</em...

By M. H. F. WILKINS, M.A., PH.D. AND S. DE CARVALHO, M.D. H EME COMPOUNDS have relatively sharp absorption bands ins the visible spectrum w’hich have beers much used to identify heme enzymes ins tissue sections, e.g. the classical work of Keilins.tm These bands are, however, not sufficiently strong for consvensienst estimations of the small amounts of heme in a single cell. The Soret band, at t...

Aim This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative to conventional polymerase chain reaction (PCR) for the detection of herpesvirus of turkeys (HVT) (FC 126 strain) in vaccinated and non-vaccinated poultry in Nigeria. Materials and Methods HVT positive control (vaccine) was used for optimization of LAMP using six primer...