Silk cocoons are composed of fiber proteins (fibroins) and adhesive glue proteins (sericins), which provide a physical barrier to protect the inside pupa. Moreover, other proteins were identified in the cocoon silk, many of which are immune related proteins. In this study, we extracted proteins from the silkworm cocoon by Tris-HCl buffer (pH7.5), and found that they had a strong inhibitory acti...

The treatment of skin infections is one the most challenging conditions in these days. Clinically health professionals recommend antibiotics; however, their efficiency questionable due to side effects, as well drug delivery capabilities. This work focuses on noninvasive application Ciprofloxacin HCL (CFx)-loaded β-Cyclodextrin composite suppress antibacterial infections. Silk fibroins are used ...

Spider mites are a group of arachnids belonging to Acari (mites and ticks), family Tetranychidae, known produce nanoscale silk fibers characterized by high Young's modulus. The fibroin gene spider has been computationally predicted through genomic analysis Tetranychus urticae Koch, but it yet be confirmed proteomic evidence. In this work, we sequenced assembled the transcriptome from two genera...

Raman microspectroscopy has been used for the first time to determine quantitatively the orientation of the beta-sheets in silk monofilaments from Bombyx mori and Samia cynthia ricini silkworms, and from the spider Nephila edulis. It is shown that, for systems with uniaxial symmetry such as silk, it is possible to determine the order parameters P2 and P4 of the orientation distribution function...

The golden silk spun by Indian golden silkmoth Antheraea assama, is regarded for its shimmering golden luster, tenacity and value as biomaterial. This report describes the gene coding for golden silk H-fibroin (AaFhc), its expression, full-length sequence and structurally important motifs discerning the underlying genetic and biochemical factors responsible for its much sought-after properties....

In the past few years a number of reviews, books and journal articles featuring silks have been published. Silks are produced by over 41,000 species of spiders (class Arachnida) and by many insects (terrestrial and aquatic), particularly in the order Lepidoptera (Foelix, 1996); they are defined as externally spun fibrous material generated from protein secretions. The cocoon silk from the domes...

Bombyx mori cocoon has a multi-layer structure that provides optimal protection for silkworm pupa. Research on the mechanical properties of the multi-layer structure revealed structure-property relationships of the cocoon. Here, we investigated the protein components of the B. mori cocoon in terms of its multi-layer structure. Liquid chromatography-tandem mass spectrometry identified 286 protei...

While fibroin isolated from the cocoons of domesticated silkworm Bombyx mori supports growth of human corneal limbal epithelial (HLE) cells, the mechanism of cell attachment remains unclear. In the present study we sought to enhance the attachment of HLE cells to membranes of Bombyx mori silk fibroin (BMSF) through surface functionalization with an arginine-glycine-aspartic acid (RGD)-containin...